Macromolecular NMR spectroscopy for the non-spectroscopist

Kwan, Ann H., Mobli, Mehdi, Gooley, Paul R., King, Glenn F. and Mackay, Joel P. (2011) Macromolecular NMR spectroscopy for the non-spectroscopist. Febs Journal, 278 5: 687-703. doi:10.1111/j.1742-4658.2011.08004.x


Author Kwan, Ann H.
Mobli, Mehdi
Gooley, Paul R.
King, Glenn F.
Mackay, Joel P.
Title Macromolecular NMR spectroscopy for the non-spectroscopist
Journal name Febs Journal   Check publisher's open access policy
ISSN 1742-464X
0945-5795
Publication date 2011-03-01
Year available 2011
Sub-type Article (original research)
DOI 10.1111/j.1742-4658.2011.08004.x
Open Access Status Not yet assessed
Volume 278
Issue 5
Start page 687
End page 703
Total pages 17
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract NMR spectroscopy is a powerful tool for studying the structure, function and dynamics of biological macromolecules. However, non-spectroscopists often find NMR theory daunting and data interpretation nontrivial. As the first of two back-to-back reviews on NMR spectroscopy aimed at non-spectroscopists, the present review first provides an introduction to the basics of macromolecular NMR spectroscopy, including a discussion of typical sample requirements and what information can be obtained from simple NMR experiments. We then review the use of NMR spectroscopy for determining the 3D structures of macromolecules and examine how to judge the quality of NMR-derived structures.
Keyword HSQC spectroscopy
Nuclear magnetic resonance (NMR) spectroscopy
Protein folding
Protein NMR spectroscopy
Protein stability
Protein structure determination
TROSY
Q-Index Code CX
Q-Index Status Confirmed Code
Grant ID DP0774245
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
ERA White List Items
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 07 Oct 2011, 23:48:36 EST by Professor Glenn King on behalf of School of Chemistry & Molecular Biosciences