Branchial osmoregulation in the euryhaline bull shark, Carcharhinus leucas: A molecular analysis of ion transporters

Reilly, Beau D., Cramp, Rebecca L., Wilson, Jonathan M., Campbell, Hamish A. and Franklin, Craig E. (2011) Branchial osmoregulation in the euryhaline bull shark, Carcharhinus leucas: A molecular analysis of ion transporters. The Journal of Experimental Biology, 214 17: 2883-2895. doi:10.1242/jeb.058156

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Author Reilly, Beau D.
Cramp, Rebecca L.
Wilson, Jonathan M.
Campbell, Hamish A.
Franklin, Craig E.
Title Branchial osmoregulation in the euryhaline bull shark, Carcharhinus leucas: A molecular analysis of ion transporters
Formatted title
Branchial osmoregulation in the euryhaline bull shark, Carcharhinus leucas: A molecular analysis of ion transporters
Journal name The Journal of Experimental Biology   Check publisher's open access policy
ISSN 0022-0949
1477-9145
Publication date 2011-09-01
Sub-type Article (original research)
DOI 10.1242/jeb.058156
Open Access Status File (Publisher version)
Volume 214
Issue 17
Start page 2883
End page 2895
Total pages 13
Place of publication Cambridge, U.K.
Publisher The Company of Biologists
Language eng
Formatted abstract
Bull sharks, Carcharhinus leucas, are one of only a few species of elasmobranchs that live in both marine and freshwater environments. Osmoregulation in euryhaline elasmobranchs is achieved through the control and integration of various organs (kidney, rectal gland and liver) in response to changes in environmental salinity. However, little is known regarding the mechanisms of ion transport in the gills of euryhaline elasmobranchs and how they are affected by osmoregulatory challenges. This study was conducted to gain insight into the branchial ion and acid-base regulatory mechanisms of C. leucas by identifying putative ion transporters and determining whether their expression is influenced by environmental salinity. We hypothesised that expression levels of the Na+/K+-ATPase (NKA) pump, Na+/H+ exchanger 3 (NHE3), vacuolar-type H+-ATPase (VHA) and anion exchanger pendrin (PDN) would be upregulated in freshwater (FW) C. leucas. Immunohistochemistry was used to localise all four ion transporters in gills of bull sharks captured in both FW and estuarine/seawater (EST/SW) environments. NHE3 immunoreactivity occurred in the apical region of cells with basolateral NKA expression whereas PDN was apically expressed in cells that also exhibited basolateral VHA immunoreactivity. In accordance with our hypotheses, quantitative real-time PCR showed that the mRNA expression of NHE3 and NKA was significantly upregulated in gills of FW-captured C. leucas relative to EST/SW-captured animals. These data suggest that NHE3 and NKA together may be important in mediating branchial Na+ uptake in freshwater environments, whereas PDN and VHA might contribute to Cl/HCO3 transport in marine and freshwater bull shark gills.
Keyword NHE3
Elasmobranch
Euryhaline
Dogfish squalus-acanthias
Mitochondria-rich cells
V-H+-ATPase
Vacuolar-type ATPase
Tilapia oreochromis-mossambicus
Phospholemman-like protein
Stingray dasyatis-sabina
Zebrafish danio-rerio
Pendred-syndrome gene
Acid-base regulation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biological Sciences Publications
 
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