Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy

Roberts, Michael S., Dancik, Yuri, Prow, Tarl W., Thorling, Camilla A., Lin, Lynlee L., Grice, Jeffrey E., Robertson, Thomas A., Koenig, Karsten and Becker, Wolfgang (2011) Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy. European Journal of Pharmaceutics and Biopharmaceutics, 77 3: 469-488. doi:10.1016/j.ejpb.2010.12.023


Author Roberts, Michael S.
Dancik, Yuri
Prow, Tarl W.
Thorling, Camilla A.
Lin, Lynlee L.
Grice, Jeffrey E.
Robertson, Thomas A.
Koenig, Karsten
Becker, Wolfgang
Title Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy
Journal name European Journal of Pharmaceutics and Biopharmaceutics   Check publisher's open access policy
ISSN 0939-6411
Publication date 2011-04-01
Year available 2011
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.ejpb.2010.12.023
Open Access Status Not yet assessed
Volume 77
Issue 3
Start page 469
End page 488
Total pages 20
Place of publication Amstedam, AE, Netherlands
Publisher Elsevier BV
Language eng
Subject 1305 Biotechnology
3003 Pharmaceutical Science
Abstract New multiphoton and confocal microscope technologies and fluorescence lifetime imaging techniques are now being used to non-invasively image, in space (three dimensions),in time, in spectra, in lifetime and in fluorescence anisotropy (total of 7 dimensions), fluorescent molecules in in situ and in vivo biological tissue, including skin. The process involves scanning a 2D area and measuring fluorescence at a given tissue depth below the surface after excitation by a laser beam with a wavelength within the one-photon or two-photon absorption band of the fluorophores followed by the stacking together of a series of 2D images from different depths to reconstruct the full spatial structure of the sample. Our aim in this work is to describe the principles, opportunities, limitations and applications of this new technology and its application in defining skin morphology, disease and skin penetration in vitro and in vivo by drugs, chemicals and nanoparticles. A key emphasis is in the use of fluorescence lifetime imaging to add additional specificity and quantitation to the detection of the various exogenous chemicals and nanoparticles that may be applied to the skin as well as endogenous fluorescent species in the skin. Examples given include equipment configuration; components in skin autofluorescence in various skin strata; imaging and quantification of coexisting drugs and their metabolites; skin pH; nanoparticle zinc oxide skin penetration; liposome delivery of drugs to deeper tissues; and observations in skin ageing and in various skin diseases.
Keyword Pharmacology & Pharmacy
Pharmacology & Pharmacy
PHARMACOLOGY & PHARMACY
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2012 Collection
School of Medicine Publications
UQ Diamantina Institute Publications
 
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Created: Wed, 28 Sep 2011, 21:57:05 EST by Matthew Lamb on behalf of School of Medicine