Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth

Windus, Louisa C. E., Chehrehasa, Fatemeh, Lineburg, Katie E., Claxton, Christina, Mackay-Sim, Alan, Key, Brian and St John, James A. (2011) Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth. Cellular and Molecular Life Sciences, 68 19: 3233-3247. doi:10.1007/s00018-011-0630-9

Author Windus, Louisa C. E.
Chehrehasa, Fatemeh
Lineburg, Katie E.
Claxton, Christina
Mackay-Sim, Alan
Key, Brian
St John, James A.
Title Stimulation of olfactory ensheathing cell motility enhances olfactory axon growth
Journal name Cellular and Molecular Life Sciences   Check publisher's open access policy
ISSN 1420-682X
Publication date 2011-10-01
Year available 2011
Sub-type Article (original research)
DOI 10.1007/s00018-011-0630-9
Open Access Status
Volume 68
Issue 19
Start page 3233
End page 3247
Total pages 15
Place of publication Switzerland
Publisher Birkhaeuser Verlag AG
Language eng
Abstract Axons of primary olfactory neurons are intimately associated with olfactory ensheathing cells (OECs) from the olfactory epithelium until the final targeting of axons within the olfactory bulb. However, little is understood about the nature and role of interactions between OECs and axons during development of the olfactory nerve pathway. We have used high resolution time-lapse microscopy to examine the growth and interactions of olfactory axons and OECs in vitro. Transgenic mice expressing fluorescent reporters in primary olfactory axons (OMP-ZsGreen) and ensheathing cells (S100ß-DsRed) enabled us to selectively analyse these cell types in explants of olfactory epithelium. We reveal here that rather than providing only a permissive substrate for axon growth, OECs play an active role in modulating the growth of pioneer olfactory axons.We show that the interactions between OECs and axons were dependent on lamellipodial waves on the shaft of OEC processes. The motility of OECs was mediated by GDNF, which stimulated cell migration and increased the apparent motility of the axons, whereas loss of OECs via laser ablation of the cells inhibited olfactory axon outgrowth. These results demonstrate that the migration of OECs strongly regulates the motility of axons and that stimulation of OEC motility enhances axon extension and growth cone activity.
Keyword GDNF
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 511006
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 28 Sep 2011, 21:06:23 EST by Professor Brian Key on behalf of School of Biomedical Sciences