Quantification of butyrylCoA:acetateCoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age

Hippe, Berit, Zwielehner, Jutta, Liszt, Kathrin, Lassl, Cornelia, Unger, Frank and Haslberger, Alexander G. (2011) Quantification of butyrylCoA:acetateCoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age. FEMS Microbiology Letters, 316 2: 130-135. doi:10.1111/j.1574-6968.2010.02197.x


Author Hippe, Berit
Zwielehner, Jutta
Liszt, Kathrin
Lassl, Cornelia
Unger, Frank
Haslberger, Alexander G.
Title Quantification of butyrylCoA:acetateCoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
1574-6968
Publication date 2011-03-01
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.2010.02197.x
Open Access Status
Volume 316
Issue 2
Start page 130
End page 135
Total pages 6
Editor Rustam Aminov
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Language eng
Abstract The gastrointestinal microbiota produces short-chain fatty acids, especially butyrate, which affect colonic health, immune function and epigenetic regulation. To assess the effects of nutrition and aging on the production of butyrate, the butyryl-CoA:acetate CoA-transferase gene and population shifts of Clostridium clusters lV and XlVa, the main butyrate producers, were analysed. Faecal samples of young healthy omnivores (24 ± 2.5 years), vegetarians (26 ± 5 years) and elderly (86 ± 8 years) omnivores were evaluated. Diet and lifestyle were assessed in questionnaire-based interviews. The elderly had significantly fewer copies of the butyryl-CoA:acetate CoA-transferase gene than young omnivores (P=0.014), while vegetarians showed the highest number of copies (P=0.048). The thermal denaturation of the butyryl-CoA:acetate CoA-transferase gene variant melting curve related to Roseburia/Eubacterium rectale spp. was significantly more variable in the vegetarians than in the elderly. The Clostridium cluster XIVa was more abundant in vegetarians (P=0.049) and in omnivores (P<0.01) than in the elderly group. Gastrointestinal microbiota of the elderly is characterized by decreased butyrate production capacity, reflecting increased risk of degenerative diseases. These results suggest that the butyryl-CoA:acetate CoA-transferase gene is a valuable marker for gastrointestinal microbiota function.
Keyword Butyric acid
Elderly
Gut microbiota
Nutrition
QPCR
Vegetarian
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Centre for Nutrition and Food Sciences Publications
 
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Created: Wed, 28 Sep 2011, 19:34:11 EST by Jutta Zwielehner on behalf of Centre for Nutrition and Food Sciences