Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture

Arifin, Yalun, Sabri, Suriana, Sugiarto, Haryadi, Krömer, Jens O., Vickers, Claudia E. and Nielsen, Lars K. (2011) Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture. Journal of Biotechnology, 156 4: 275-278. doi:10.1016/j.jbiotec.2011.07.003

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Author Arifin, Yalun
Sabri, Suriana
Sugiarto, Haryadi
Krömer, Jens O.
Vickers, Claudia E.
Nielsen, Lars K.
Title Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
Formatted title
Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture
Journal name Journal of Biotechnology   Check publisher's open access policy
ISSN 0168-1656
1873-4863
Publication date 2011-12-01
Sub-type Article (original research)
DOI 10.1016/j.jbiotec.2011.07.003
Volume 156
Issue 4
Start page 275
End page 278
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture.
Keyword Sucrose
Escherachia coli W
Poly-3-hydroxybutyrate
Fermentation
Fed-batch
Industrial biotechnology
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Special issue: IBS 2010 - Industrial Biotechnology

 
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Created: Tue, 20 Sep 2011, 23:26:23 EST by Dr Claudia Vickers on behalf of Aust Institute for Bioengineering & Nanotechnology