Salmonella bongori provides insights into the evolution of the salmonellae

Fookes, Maria, Schroeder, Gunnar N., Langridge, Gemma C., Blondel, Carlos J., Mammina, Caterina, Connor, Thomas R., Seth-Smith, Helena, Vernikos, Georgios S., Robinson, Keith S., Sanders, Mandy, Petty, Nicola K., Kingsley, Robert A., Baumler, Andreas J., Nuccio, Sean-Paul, Contreras, Ines, Santiviago, Carlos A., Maskell, Duncan, Barrow, Paul, Humphrey, Tom, Nastasi, Antonino, Roberts, Mark, Frankel, Gad, Parkhill, Julian, Dougan, Gordon and Thomson, Nicholas R. (2011) Salmonella bongori provides insights into the evolution of the salmonellae. PLoS Pathogens, 7 8: e1002191.1-e1002191.16. doi:10.1371/journal.ppat.1002191

Author Fookes, Maria
Schroeder, Gunnar N.
Langridge, Gemma C.
Blondel, Carlos J.
Mammina, Caterina
Connor, Thomas R.
Seth-Smith, Helena
Vernikos, Georgios S.
Robinson, Keith S.
Sanders, Mandy
Petty, Nicola K.
Kingsley, Robert A.
Baumler, Andreas J.
Nuccio, Sean-Paul
Contreras, Ines
Santiviago, Carlos A.
Maskell, Duncan
Barrow, Paul
Humphrey, Tom
Nastasi, Antonino
Roberts, Mark
Frankel, Gad
Parkhill, Julian
Dougan, Gordon
Thomson, Nicholas R.
Title Salmonella bongori provides insights into the evolution of the salmonellae
Formatted title
Salmonella bongori provides insights into the evolution of the salmonellae
Journal name PLoS Pathogens   Check publisher's open access policy
ISSN 1553-7366
Publication date 2011-08-01
Sub-type Article (original research)
DOI 10.1371/journal.ppat.1002191
Open Access Status DOI
Volume 7
Issue 8
Start page e1002191.1
End page e1002191.16
Total pages 16
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Formatted abstract
The genus Salmonella contains two species, S. bongori and S. enterica. Compared to the well-studied S. enterica there is a marked lack of information regarding the genetic makeup and diversity of S. bongori. S. bongori has been found predominantly associated with cold-blooded animals, but it can infect humans. To define the phylogeny of this species, and compare it to S. enterica, we have sequenced 28 isolates representing most of the known diversity of S. bongori. This cross-species analysis allowed us to confidently differentiate ancestral functions from those acquired following speciation, which include both metabolic and virulence-associated capacities. We show that, although S. bongori inherited a basic set of Salmonella common virulence functions, it has subsequently elaborated on this in a different direction to S. enterica. It is an established feature of S. enterica evolution that the acquisition of the type III secretion systems (T3SS-1 and T3SS-2) has been followed by the sequential acquisition of genes encoding secreted targets, termed effectors proteins. We show that this is also true of S. bongori, which has acquired an array of novel effector proteins (sboA-L). All but two of these effectors have no significant S. enterica homologues and instead are highly similar to those found in  enteropathogenic Escherichia coli (EPEC). Remarkably, SboH is found to be a chimeric effector protein, encoded by a fusion of the T3SS-1 effector gene sopA and a gene highly similar to the EPEC effector nleH from enteropathogenic E. coli. We demonstrate that representatives of these new effectors are translocated and that SboH, similarly to NleH, blocks intrinsic apoptotic pathways while being targeted to the mitochondria by the SopA part of the fusion. This work suggests that S. bongori has inherited the ancestral Salmonella virulence gene set, but has adapted by incorporating virulence determinants that resemble those employed by EPEC.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Article no.e1002191.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 46 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 53 times in Scopus Article | Citations
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Created: Tue, 20 Sep 2011, 22:08:01 EST by Dr Nicola Petty on behalf of School of Chemistry & Molecular Biosciences