Relationship between semen quality parameters and field fertility of young dairy bulls using low dose inseminations

Christensen, P,., Labouriau, R., Birck, A., Boe-Hansen, G.B., Pedersen, J. and Borchersen, S. (2011) Relationship between semen quality parameters and field fertility of young dairy bulls using low dose inseminations. Journal of Dairy Science, 94 4: 1744-1754. doi:10.3168/jds.2010-3087

Author Christensen, P,.
Labouriau, R.
Birck, A.
Boe-Hansen, G.B.
Pedersen, J.
Borchersen, S.
Title Relationship between semen quality parameters and field fertility of young dairy bulls using low dose inseminations
Journal name Journal of Dairy Science   Check publisher's open access policy
ISSN 0022-0302
Publication date 2011-01-01
Year available 2011
Sub-type Article (original research)
DOI 10.3168/jds.2010-3087
Open Access Status Not yet assessed
Volume 94
Issue 4
Start page 1744
End page 1754
Total pages 11
Place of publication United States
Publisher Elsevier Inc.
Language eng
Subject 1106 Food Science
1103 Animal Science and Zoology
1311 Genetics
Abstract Optimal use of genetically superior bulls through artificial insemination (AI) is highly dependent on precise assessment of seminal quality which allows for reasonable estimations of field fertility with normal or low-dose inseminations. In the present study, seminal measures such as sperm motility and morphology, sperm viability, sperm DNA fragmentation, and the ability of the sperm to display an acrosome reaction were tested. The relationships between field fertility and the seminal measures were investigated using 3 ejaculates from each of 195 bulls (156 Holstein and 39 Jersey) participating in a progeny test program. A range of AI doses, varying from 2 × 106 to 15 × 106 sperm/ straw, was obtained by a controlled dilution process applied to each ejaculate. The different AI doses were distributed at random among 75,610 experimental first inseminations in 4,721 herds and 208 AI technicians. Most of the seminal measures appeared to contain a predictive value for the nonreturn to estrus at 56 d post-AI (NRR56) regardless of the number of sperm per AI dose and can be regarded as noncompensable sperm traits. But, due to correlations between the individual measures, the best model for describing (and predicting) NRR56 was based on sperm concentration and viability in the neat (raw) semen, and post-thaw sperm viability. The statistical models for describing NRR56 included the following explanatory variables: strength of the estrus, number of sperm per AI dose, breed, parity, and random components representing herds and AI technicians. The present results show that the most precise estimation of a bull’s NRR56 can be achieved through flow cytometric detection of sperm concentration and viability in neat semen as well as flow cytometric detection of post-thaw sperm viability.
Keyword Flow cytometry
DNA fragmentation
Acrosome reaction
Sperm viability
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 93S-2465-Angstrom00-1120
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
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Created: Sat, 17 Sep 2011, 01:07:19 EST by Dr Gry Boe-hansen on behalf of School of Veterinary Science