Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential

Frith, Jessica E., Thomson, Brian and Genever, Paul G. (2010) Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential. Tissue Engineering Part C: Methods, 16 4: 735-749. doi:10.1089/ten.tec.2009.0432


Author Frith, Jessica E.
Thomson, Brian
Genever, Paul G.
Title Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential
Journal name Tissue Engineering Part C: Methods   Check publisher's open access policy
ISSN 1937-3384
1937-3392
Publication date 2010-07-23
Year available 2009
Sub-type Article (original research)
DOI 10.1089/ten.tec.2009.0432
Open Access Status Not yet assessed
Volume 16
Issue 4
Start page 735
End page 749
Total pages 15
Place of publication New Rochelle, NY, U.S.A.
Publisher Mary Ann Liebert Publishers
Language eng
Formatted abstract
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation along the osteogenic, chondrogenic, and adipogenic lineages and have potential applications in a range of therapies. MSCs can be cultured as monolayers on tissue culture plastic, but there are indications that they lose cell-specific properties with time in vitro and so poorly reflect in vivo MSC behavior. We developed dynamic three-dimensional (3D) techniques for in vitro MSC culture using spinner flasks and a rotating wall vessel bioreactor. We characterized the two methods for dynamic 3D MSC culture and compared the properties of these cultures with monolayer MSCs. Our results showed that under optimal conditions, MSCs form compact cellular spheroids and remain viable in dynamic 3D culture. We demonstrated altered cell size and surface antigen expression together with enhanced osteogenic and adipogenic differentiation potential in MSCs from dynamic 3D conditions. By microarray analysis of monolayer and spinner flask MSCs, we identified many differences in gene expression, including those confirming widespread changes to the cellular architecture and extracellular matrix. The upregulation of interleukin 24 in dynamic 3D cultures was shown to selectively impair the viability of prostate cancer cells cultured in medium conditioned by dynamic 3D MSCs. Overall, this work suggests a novel therapeutic application for dynamic 3D MSCs and demonstrates that these methods are a viable alternative to monolayer techniques and may prove beneficial for retaining MSC properties in vitro.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Online Ahead of Editing: October 7, 2009

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Mon, 12 Sep 2011, 19:21:37 EST by Dr Jessica Frith on behalf of Aust Institute for Bioengineering & Nanotechnology