Loop-mediated isothermal amplification test for Trypanosoma vivax based on satellite repeat DNA

Njiru, Z. K., Ouma, J. O., Bateta, R., Njeru, S. E., Ndungu, K., Gitonga, P. K., Guya, S. and Traub, R. (2011) Loop-mediated isothermal amplification test for Trypanosoma vivax based on satellite repeat DNA. Veterinary Parasitology, 180 3-4: 358-362. doi:10.1016/j.vetpar.2011.03.021


Author Njiru, Z. K.
Ouma, J. O.
Bateta, R.
Njeru, S. E.
Ndungu, K.
Gitonga, P. K.
Guya, S.
Traub, R.
Title Loop-mediated isothermal amplification test for Trypanosoma vivax based on satellite repeat DNA
Formatted title
Loop-mediated isothermal amplification test for Trypanosoma vivax based on satellite repeat DNA
Journal name Veterinary Parasitology   Check publisher's open access policy
ISSN 0304-4017
1873-2550
Publication date 2011-08-25
Sub-type Article (original research)
DOI 10.1016/j.vetpar.2011.03.021
Open Access Status Not Open Access
Volume 180
Issue 3-4
Start page 358
End page 362
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
Trypanosoma vivax is major cause of animal trypanosomiasis and responsible for enormous economic burden in Africa and South America animal industry. T. vivax infections mostly run low parasitaemia with no apparent clinical symptoms, making diagnosis a challenge. This work reports the design and evaluation of a loop-mediated isothermal amplification (LAMP) test for detecting T. vivax DNA based on the nuclear satellite repeat sequence. The assay is rapid with results obtained within 35 min. The analytical sensitivity is ~1 trypanosome/ml while that of the classical PCR tests ranged from 10 to 103 trypanosomes/ml. The T. vivax LAMP test reported here is simple, robust and has future potential in diagnosis of animal trypanosomiasis in the field.
Keyword Loop-mediated isothermal amplification
Trypanosoma vivax
Nagana
Trypanosomiasis
PCR
Linked-immunosorbent-assay
Duttonella vivax
African trypanosomiasis
Sensitive detection
South-america
Tsetse-flies
Cattle
Antibodies
Infection
Elisa
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Veterinary Science Publications
 
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