Discrimination of All Members of the Anophelespunctulatus Complex by Polymerase Chain-Reaction Restriction-Fragment-Length-Polymorphism Analysis

Beebe, NW and Saul, A (1995) Discrimination of All Members of the Anophelespunctulatus Complex by Polymerase Chain-Reaction Restriction-Fragment-Length-Polymorphism Analysis. American Journal of Tropical Medicine and Hygiene, 53 5: 478-481. doi:10.4269/ajtmh.1995.53.478


Author Beebe, NW
Saul, A
Title Discrimination of All Members of the Anophelespunctulatus Complex by Polymerase Chain-Reaction Restriction-Fragment-Length-Polymorphism Analysis
Journal name American Journal of Tropical Medicine and Hygiene   Check publisher's open access policy
ISSN 0002-9637
Publication date 1995-11-01
Year available 1995
Sub-type Article (original research)
DOI 10.4269/ajtmh.1995.53.478
Open Access Status Not yet assessed
Volume 53
Issue 5
Start page 478
End page 481
Total pages 4
Place of publication MCLEAN
Publisher AMER SOC TROP MED & HYGIENE
Language eng
Abstract A method has been developed to identify the members of the Anopheles punctulatus complex using polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP). Members of the An. punctulatus complex are the most important vectors of malaria in the southwest Pacific and consist of 10 cryptic species, An. farauti no. 1-7, An. punctulatus, An. sp. near punctulatus, and An. koliensis. For each species, PCR amplification of the ribosomal DNA internal transcribed spacer produced a 750-basepair product. Digestion with Msp I and electrophoresis on a 3.0% agarose gel results in banding patterns unique to each species. Isolates of the same species from different locations gave an identical pattern. The technique is sensitive enough so that a PCR-RFLP can be generated from as little as a single mosquito leg, allowing the rest of the mosquito to be used for other important epidemiologic analyses such as determining host feeding source, and for parasite detection.
Keyword Anopheles-Punctulatus Complex
Farauti-Laveran
Culicidae
Diptera
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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