Tissue-specific soluble sugars in seeds as indicators of storage category

Steadman, KJ, Pritchard, HW and Dey, PM (1996) Tissue-specific soluble sugars in seeds as indicators of storage category. Annals of Botany, 77 6: 667-674. doi:10.1006/anbo.1996.0083


Author Steadman, KJ
Pritchard, HW
Dey, PM
Title Tissue-specific soluble sugars in seeds as indicators of storage category
Journal name Annals of Botany   Check publisher's open access policy
ISSN 0305-7364
Publication date 1996-06-01
Year available 1996
Sub-type Article (original research)
DOI 10.1006/anbo.1996.0083
Open Access Status Not yet assessed
Volume 77
Issue 6
Start page 667
End page 674
Total pages 8
Place of publication LONDON
Publisher ACADEMIC PRESS LTD
Language eng
Abstract The accumulation of specific soluble carbohydrates has been implicated in the acquisition of desiccation tolerance and improved longevity in orthodox seeds, leading to the hypothesis that carbohydrate composition might be used as a diagnostic marker for seed storage category. We have studied the sugar composition of 46 tissues from seeds of 18 species, covering 13 genera and ten families, and representing three seed storage categories: orthodox, intermediate and recalcitrant. Soluble carbohydrate extracts from each tissue were analysed using High pH Anion Exchange Chromatography. Total sugar content and sucrose level of the embryo were highly variable across all species and no simple association with seed storage physiology was evident. Monosaccharide levels were found to be low in most seeds studied, including those of the recalcitrant category. Sucrosyl-oligosaccharides, raffinose and stachyose, were observed to be lower in recalcitrant seeds compared to orthodox seeds. In general, orthodox and recalcitrant seeds had tissues with sucrosyl-oligosaccharide:sucrose mass ratios of > 0.143 (i.e. 1:7) and < 0.083 (1:12) respectively. Moreover, large variations in the content of these sugars were noted between different tissues within the recalcitrant and intermediate seeds. The results from the present study in combination with data in the literature show that the ratio of sucrosyl-oligosaccharide:sucrose in seed tissues is generally a good indicator of seed storage category. (C) 1996 Annals of Botany Company.
Keyword seed storage
desiccation tolerance
orthodox seeds
recalcitrant seeds
soluble sugars
sucrosyl-oligosaccharides
Hplc
seed tissues
Desiccation Tolerance
Recalcitrant Seeds
Water Status
Germination
Sensitivity
Acquisition
Maturation
Viability
Proteins
Behavior
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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