NeuroD2 is necessary for development and survival of central nervous system neurons

Olson, JM, Asakura, A, Snider, L, Hawkes, R, Strand, A, Stoeck, J, Hallahan, A, Pritchard, J and Tapscott, SJ (2001) NeuroD2 is necessary for development and survival of central nervous system neurons. Developmental Biology, 234 1: 174-187. doi:10.1006/dbio.2001.0245


Author Olson, JM
Asakura, A
Snider, L
Hawkes, R
Strand, A
Stoeck, J
Hallahan, A
Pritchard, J
Tapscott, SJ
Title NeuroD2 is necessary for development and survival of central nervous system neurons
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
Publication date 2001-06-01
Year available 2001
Sub-type Article (original research)
DOI 10.1006/dbio.2001.0245
Open Access Status Not yet assessed
Volume 234
Issue 1
Start page 174
End page 187
Total pages 14
Place of publication SAN DIEGO
Publisher ACADEMIC PRESS INC
Language eng
Abstract NeuroD2 is sufficient to induce cell cycle arrest and neurogenic differentiation in nonneuronal cells. To determine whether this bHLH transcription factor was necessary for normal brain development, we used homologous recombination to replace the neuroD2 coding region with a p-galactosidase reporter gene. The neuroD2 gene expressed the reporter in a subset of neurons in the central nervous system, including in neurons of the neocortex and hippocampus and cerebellum. NeuroD2(-/-)mice showed normal development until about day P14, when they began exhibiting ataxia and failure to thrive. Brain areas that expressed neuroD2 were smaller than normal and showed higher rates of apoptosis, Cerebella of neuroD2-null mice expressed reduced levels of genes encoding proteins that support cerebellar granule cell survival, including brain-derived neurotrophic factor (BDNF), Decreased levels of BDNF and higher rates of apoptosis in cerebellar granule cells of neuroDe(-/-)mice indicate that neuroD2 is necessary for the survival of specific populations of central nervous system neurons in addition to its known effects on cell cycle regulation and neuronal differentiation. (C) 2001 Academic Press.
Keyword neurogenic bHLH transcription factors
cerebellum
Bdnf
neuronal apoptosis
microarray analysis
Loop-Helix Protein
Cerebellar Granule Neurons
Phosphatidylinositol 3-Kinase
Neurotrophic Factor
Transcription Factors
Transient Expression
Determination Genes
Family
Mice
Growth
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID HD28834
NS36086
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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