Impact of multiple HPV infection on response to treatment and survival in patients receiving radical radiotherapy for cervical cancer

Bachtiary, B, Obermair, A, Dreier, B, Birner, P, Breitenecker, G, Knocke, TH, Selzer, E and Potter, R (2002) Impact of multiple HPV infection on response to treatment and survival in patients receiving radical radiotherapy for cervical cancer. International Journal of Cancer, 102 3: 237-243. doi:10.1002/ijc.10708


Author Bachtiary, B
Obermair, A
Dreier, B
Birner, P
Breitenecker, G
Knocke, TH
Selzer, E
Potter, R
Title Impact of multiple HPV infection on response to treatment and survival in patients receiving radical radiotherapy for cervical cancer
Journal name International Journal of Cancer   Check publisher's open access policy
ISSN 0020-7136
Publication date 2002-11-01
Year available 2002
Sub-type Article (original research)
DOI 10.1002/ijc.10708
Open Access Status DOI
Volume 102
Issue 3
Start page 237
End page 243
Total pages 7
Place of publication MALDEN
Publisher WILEY-BLACKWELL
Language eng
Abstract To obtain information on the incidence and the clinical significance of infection with various types of the human papillomavirus (HPV) in cancer of the uterine cervix, we retrospectively examined the HPV status of 106 patients who had received radical radiotherapy for cervical cancer stages IIB to IIIB. DNA was extracted from formalin-fixed, paraffin-embedded biopsies and PCR was carried out to identify HPV types 16, 18, 31, 35, 33 and 45. To detect additional HPV types, consensus PCR products were cloned and sequenced. A catalyzed signal-amplified colorimetric in situ hybridization was carried out in 84 of 106 specimens as a positive control. Response to therapy, progression-free survival (PFS) and cervical cancer-specific survival (CCSS) were the statistical end-points. Survival analysis was carried out using univariate and multivariate analysis (Cox regression). Ninety-six patients (90.6%) were HPV-positive and 42/96 (43.7%) were positive for multiple HPV types. Eight patients had persistent disease after radiotherapy. From these 8 patients, 7 were infected with multiple HPV types and only I patient had an infection with a single HPV type. After a median follow up period of 50 months, patients with multiple HPV infection had a significantly shorter PFS and CCSS compared to those with single HPV infection (24.8% and 34.9% vs. 64% and 60.8%, Log rank, p < 0.01 and 0.04). In multivariate analysis, the presence of multiple HPV types (RR 1.9), node status (RR 2.3), tumor size (RR 3.2) and histologic type (RR 4.8) were independent prognostic factors of CCSS. Our results demonstrate that the presence of multiple HPV types is associated with poor response and with reduced survival in cervical cancer patients who receive radiotherapy as the primary treatment. (C) 2002 Wiley-Liss, Inc.
Keyword cervical cancer
multiple HPV
radiotherapy
Human-Papillomavirus Infection
Prognostic-Significance
High-Risk
Dna
Carcinoma
Radiation
Women
Pcr
P53
Prevalence
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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