High-Performance Liquid-Chromatographic Separation of the Enantiomers of Hydroxychloroquine and its Major Metabolites in Biological-Fluids Using An Alpha-1-Acid Glycoprotein Stationary Phase

McLachlan, AJ, Tett, SE and Cutler, DJ (1991) High-Performance Liquid-Chromatographic Separation of the Enantiomers of Hydroxychloroquine and its Major Metabolites in Biological-Fluids Using An Alpha-1-Acid Glycoprotein Stationary Phase. Journal of Chromatography-Biomedical Applications, 570 1: 119-127. doi:10.1016/0378-4347(91)80206-R


Author McLachlan, AJ
Tett, SE
Cutler, DJ
Title High-Performance Liquid-Chromatographic Separation of the Enantiomers of Hydroxychloroquine and its Major Metabolites in Biological-Fluids Using An Alpha-1-Acid Glycoprotein Stationary Phase
Journal name Journal of Chromatography-Biomedical Applications
ISSN 0378-4347
Publication date 1991-09-01
Year available 1991
Sub-type Article (original research)
DOI 10.1016/0378-4347(91)80206-R
Open Access Status Not yet assessed
Volume 570
Issue 1
Start page 119
End page 127
Total pages 9
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract An enantioselective two-stage off-line assay has been developed for the analysis of hydroxychloroquine and its three major metabolites in biological fluids. The first non-stereoselective stage of the assay (PRP-1 column) separates and quantitates parent drug and metabolites. Fractions containing hydroxychloroquine and each of the metabolites are collected manually, evaporated, reconstituted in mobile phase and re-injected onto an alpha-1-acid glycoprotein column to separate and determine proportions of individual enantiomers. Preliminary results from patient samples indicate that the disposition of hydroxychloroquine and its major metabolites is enantioselective.
Keyword Chloroquine Enantiomers
Racemic Drugs
Resolution
Desethylchloroquine
Derivatives
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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