Axonal and dendritic endocytic pathways in cultured neurons

Parton, R. G., Simons, K. and Dotti, C. G. (1992) Axonal and dendritic endocytic pathways in cultured neurons. Journal of Cell Biology, 119 1: 123-137. doi:10.1083/jcb.119.1.123

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Author Parton, R. G.
Simons, K.
Dotti, C. G.
Title Axonal and dendritic endocytic pathways in cultured neurons
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 1992-10-01
Year available 1992
Sub-type Article (original research)
DOI 10.1083/jcb.119.1.123
Open Access Status File (Publisher version)
Volume 119
Issue 1
Start page 123
End page 137
Total pages 15
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract The endocytic pathways from the axonal and dendritic surfaces of cultured polarized hippocampal neurons were examined. The dendrites and cell body contained extensive networks of tubular early endosomes which received endocytosed markers from the somatodendritic domain. In axons early endosomes were confined to presynaptic terminals and to varicosities. The somatodendritic but not the presynaptic early endosomes were labeled by internalized transferrin. In contrast to early endosomes, late endosomes and lysosomes were shown to be predominantly located in the cell body. Video microscopy was used to follow the transport-t of internalized markers from the periphery of axons and dendrites back to the cell body. Labeled structures in both domains moved unidirectionally by retrograde fast transport. Axonally transported organelles were sectioned for EM after video microscopic observation and shown to be large multivesicular body-like structures. Similar structures accumulated at the distal side of an axonal lesion. Multivesicular bodies therefore appear to be the major structures mediating transport of endocytosed markers between the nerve terminals and the cell body. Late endocytic structures were also shown to be highly mobile and were observed moving within the cell body and proximal dendritic segments. The results show that the organization of the endosomes differs in the axons and dendrites of cultured rat hippocampal neurons and that the different compartments or stages of the endocytic pathways can be resolved spatially.
Keyword Mannose 6-phosphate receptor
Synaptic vesicle membrane
Wheat-germ agglutinin
Hippocampal-neurons
Tetanus toxin
Transferrin receptors
Lysosomal membrane
Early endosomes
Fusion invitro
Transport
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biomedical Sciences Publications
Institute for Molecular Bioscience - Publications
 
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