Involvement of the transmembrane protein p23 in biosynthetic protein transport

Rojo, M., Pepperkok, R., Emery, G., Kellner, R., Stang, E., Parton, R. G. and Gruenberg, J. (1997) Involvement of the transmembrane protein p23 in biosynthetic protein transport. Journal of Cell Biology, 139 5: 1119-1135. doi:10.1083/jcb.139.5.1119

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Author Rojo, M.
Pepperkok, R.
Emery, G.
Kellner, R.
Stang, E.
Parton, R. G.
Gruenberg, J.
Title Involvement of the transmembrane protein p23 in biosynthetic protein transport
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 1997-12-01
Year available 1997
Sub-type Article (original research)
DOI 10.1083/jcb.139.5.1119
Open Access Status File (Publisher version)
Volume 139
Issue 5
Start page 1119
End page 1135
Total pages 17
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract The identification of ANO1/TMEM16A as the likely calcium-dependent chloride channel of exocrine glands has led to a more detailed understanding of its biophysical properties. This includes a calcium-dependent change in channel selectivity and evidence that HCO3 (-) permeability can be significant. Here we use freshly isolated pancreatic acini that preserve the luminal structure to measure intraluminal pH and test the idea that ANO1/TMEM16A contributes to luminal pH balance. Our data show that, under physiologically relevant stimulation with 10 pm cholesystokinin, the luminal acid load that results from the exocytic fusion of zymogen granules is significantly blunted by HCO3 (-) buffer in comparison with HEPES, and that this is blocked by the specific TMEM16A inhibitor T16inh-A01. Furthermore, in a model of acute pancreatitis, we observed substantive luminal acidification and provide evidence that ANO1/TMEM16A acts to attenuate this pH shift. We conclude that ANO1/TMEM16A is a significant pathway in pancreatic acinar cells for HCO3 (-) secretion into the lumen.
Keyword Integral membrane-protein
Endoplasmic-reticulum membrane
Stomatitis-virus glycoprotein
Copii-coated vesicles
Cis-golgi network
Intermediate compartment
Brefeldin-a
Beta-cop
Endocytic pathway
Cell-surface
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biomedical Sciences Publications
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 120 times in Thomson Reuters Web of Science Article | Citations
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