Sphingolipid Transport From the Transgolgi Network to the Apical Surface in Permeabilized Mdck Cells

Kobayashi, T, Pimplikar, SW, Parton, RG, Bhakdi, S and Simons, K (1992) Sphingolipid Transport From the Transgolgi Network to the Apical Surface in Permeabilized Mdck Cells. Febs Letters, 300 3: 227-231. doi:10.1016/0014-5793(92)80851-7


Author Kobayashi, T
Pimplikar, SW
Parton, RG
Bhakdi, S
Simons, K
Title Sphingolipid Transport From the Transgolgi Network to the Apical Surface in Permeabilized Mdck Cells
Journal name Febs Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 1992-04-01
Year available 1992
Sub-type Article (original research)
DOI 10.1016/0014-5793(92)80851-7
Open Access Status Not yet assessed
Volume 300
Issue 3
Start page 227
End page 231
Total pages 5
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract We have measured the transport of de novo synthesized fluorescent analogs of sphingomyelin and glucosylceramide from the trans-Golgi network (TGN) to the apical membrane in basolaterally permeabilized Madin-Darby canine kidney (MDCK) cells. Sphingolipid transport was temperature, ATP and cytosol dependent. Introduction of bovine serum albumin (BSA), which binds fluorescent sphingolipid monomer, into the permeabilized cells, did not affect lipid transport to the apical membrane. Both fluorescent sphingomyelin and glucosylceramide analogs were localized to the lumenal bilayer leaflet of isolated TGN-derived vesicles. These results strongly suggest that both sphingolipids are transported from the TGN to the apical membrane via vesicular traffic.
Keyword Sphingolipid Transport
Fluorescent Lipid Analog
Streptolysin-O
Transgolgi Network
Permeabilized Cell
Apical Membrane
Mdck Cell
Plasma-Membrane
Streptolysin-O
Sphingomyelin
Apparatus
Vesicles
Glycoprotein
Delivery
Proteins
Occurs
Cis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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