VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles

Kurzchalia, T. V., Dupree, P., Parton, R. G., Kellner, R., Virta, H., Lehnert, M. and Simons, K. (1992) VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles. Journal of Cell Biology, 118 5: 1003-1014. doi:10.1083/jcb.118.5.1003

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Author Kurzchalia, T. V.
Dupree, P.
Parton, R. G.
Kellner, R.
Virta, H.
Lehnert, M.
Simons, K.
Title VIP21, a 21-kD membrane protein is an integral component of trans-Golgi-network-derived transport vesicles
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 1992-09-01
Year available 1992
Sub-type Article (original research)
DOI 10.1083/jcb.118.5.1003
Open Access Status File (Publisher version)
Volume 118
Issue 5
Start page 1003
End page 1014
Total pages 12
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract In simple epithelial cells, apical and basolateral proteins are sorted into separate vesicular carriers before delivery to the appropriate plasma membrane domains. To dissect the putative sorting machinery, we have solubilized Golgi-derived transport vesicles with the detergent CHAPS and shown that an apical marker, influenza haemagglutinin (HA), formed a large complex together with several integral membrane proteins. Remarkably, a similar set of CHAPS-insoluble proteins was found after solubilization of a total cellular membrane fraction. This allowed the cloning of a cDNA encoding one protein of this complex, VIP21 (Vesicular Integral-membrane Protein of 21 kD). The transiently expressed protein appeared on the Golgi-apparatus, the plasma membrane and vesicular structures. We propose that VIP21 is a component of the molecular machinery of vesicular transport.
Keyword Polarized epithelial-cells
Canine kidney-cells
Plasma-membrane
Endoplasmic-reticulum
Secretory vesicles
Sequence-analysis
Coated vesicles
Influenza-virus
Mammalian-cells
Mdck cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biomedical Sciences Publications
Institute for Molecular Bioscience - Publications
 
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