Rab8, a small GTPase involved in vesicular traffic between the TGN and the basolateral plasma membrane

Huber, L. A., Pimplikar, S., Parton, R. G., Virta, H., Zerial, M. and Simons, K. (1993) Rab8, a small GTPase involved in vesicular traffic between the TGN and the basolateral plasma membrane. Journal of Cell Biology, 123 1: 35-45. doi:10.1083/jcb.123.1.35

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Author Huber, L. A.
Pimplikar, S.
Parton, R. G.
Virta, H.
Zerial, M.
Simons, K.
Title Rab8, a small GTPase involved in vesicular traffic between the TGN and the basolateral plasma membrane
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 1993-10-01
Year available 1993
Sub-type Article (original research)
DOI 10.1083/jcb.123.1.35
Open Access Status File (Publisher version)
Volume 123
Issue 1
Start page 35
End page 45
Total pages 11
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract Small GTP-binding proteins of the rab family have been implicated as regulators of membrane traffic along the biosynthetic and endocytic pathways in eukaryotic cells. We have investigated the localization and function of rab8, closely related to the yeast YPT1/SEC4 gene products. Confocal immunofluorescence microscopy and immunoelectron microscopy on filter-grown MDCK cells demonstrated that, rab8 was localized to the Golgi region, vesicular structures, and to the basolateral plasma membrane. Two-dimensional gel electrophoresis showed that rab8p was highly enriched in immuno-isolated basolateral vesicles carrying vesicular stomatitis virus-glycoprotein (VSV-G) but was absent from vesicles transporting the hemagglutinin protein (HA) of influenza virus to the apical cell surface.
Keyword Adp-ribosylation factor
Gdp/gtp exchange protein
Trans-golgi-network
Canine kidney-cells
Binding-proteins
Mdck cells
Molecular-cloning
Stomatitis-virus
Epithelial-cells
Endosome fusion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biomedical Sciences Publications
Institute for Molecular Bioscience - Publications
 
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