Ultrastructural-Localization of Gangliosides - Gm(1) Is Concentrated in Caveolae

Parton, R. G. (1994) Ultrastructural-Localization of Gangliosides - Gm(1) Is Concentrated in Caveolae. Journal of Histochemistry & Cytochemistry, 42 2: 155-166. doi:10.1177/42.2.8288861


Author Parton, R. G.
Title Ultrastructural-Localization of Gangliosides - Gm(1) Is Concentrated in Caveolae
Journal name Journal of Histochemistry & Cytochemistry   Check publisher's open access policy
ISSN 0022-1554
Publication date 1994-02-01
Year available 1994
Sub-type Other
DOI 10.1177/42.2.8288861
Open Access Status Not yet assessed
Volume 42
Issue 2
Start page 155
End page 166
Total pages 12
Place of publication LONDON
Publisher SAGE PUBLICATIONS LTD
Language eng
Abstract The ultrastructural distribution of the ganglioside GM(1) was investigated in A431 cells. After fixation, the cells were frozen in liquid nitrogen, freeze-substituted, and then embedded in Lowicryl resin at -45 degrees C. By use of the cholera toxin-binding subunit adsorbed to gold as a specific probe to label on the sections, GM(1) was shown to be present in endocytic organelles, in the trans-Golgi network, and on the plasma membrane, but was not detectable in the endoplasmic reticulum. GM(1) was not distributed uniformly over the plasma membrane but was concentrated approximately four-fold in non-coated invaginations. These were identified as caveolae by labeling frozen sections of cholera toxin-gold surface-labeled cells with antibodies to VIP-21/caveolin. The results strengthen the functional analogy between caveolae and sorting domains of the TGN in polarized epithelial cells.
Keyword Electron Microscopy
Stereology
Freeze-Substitution
Colloidal Gold
Ganglioside
Caveolae
Polarized Epithelial-Cells
Cholera-Toxin
Anchored Proteins
Gm1
Transport
Component
Tyrosine
Receptor
Internalization
Invaginations
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Other
Collection: Institute for Molecular Bioscience - Publications
 
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