The Small Gtpase Rab5 Functions as a Regulatory Factor in the Early Endocytic Pathway

Bucci, C, Parton, RG, Mather, IH, Stunnenberg, H, Simons, K, Hoflack, B and Zerial, M (1992) The Small Gtpase Rab5 Functions as a Regulatory Factor in the Early Endocytic Pathway. Cell, 70 5: 715-728. doi:10.1016/0092-8674(92)90306-W


Author Bucci, C
Parton, RG
Mather, IH
Stunnenberg, H
Simons, K
Hoflack, B
Zerial, M
Title The Small Gtpase Rab5 Functions as a Regulatory Factor in the Early Endocytic Pathway
Journal name Cell   Check publisher's open access policy
ISSN 0092-8674
Publication date 1992-09-01
Year available 1992
Sub-type Article (original research)
DOI 10.1016/0092-8674(92)90306-W
Open Access Status Not yet assessed
Volume 70
Issue 5
Start page 715
End page 728
Total pages 14
Place of publication CAMBRIDGE
Publisher CELL PRESS
Language eng
Abstract We have investigated the in vivo functional role of rab5, a small GTPase associated with the plasma membrane and early endosomes. Wild-type rab5 or rab5ile133, a mutant protein defective in GTP binding, was overexpressed in baby hamster kidney cells. In cells expressing the rab5ile133 protein, the rate of endocytosis was decreased by 50% compared with normal, while the rate of recycling was not significantly affected. The morphology of early endosomes was also drastically changed by the mutant protein, which induced accumulation of small tubules and vesicles at the periphery of the cell. Surprisingly, overexpression of wild-type rab5 accelerated the uptake of endocytic markers and led to the appearance of atypically large early endosomes. We conclude that rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
Keyword Cell-Free System
Receptor-Mediated Endocytosis
Recombinant Vaccinia Virus
Binding Ypt1 Protein
Coated Vesicles
Endoplasmic-Reticulum
Transferrin Receptors
Vesicular Transport
Golgi Transport
Fusion Invitro
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1025 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 964 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 10 Sep 2011, 05:56:58 EST by System User