Relationship between alcohol intake and immunoglobulin A immunoreactivity with acetaldehyde-modified bovine serum albumin

Worrall, S, deJersey, J, Wilce, PA, Seppa, K, Hurme, L and Sillanaukee, P (1996) Relationship between alcohol intake and immunoglobulin A immunoreactivity with acetaldehyde-modified bovine serum albumin. Alcoholism-Clinical and Experimental Research, 20 5: 836-840. doi:10.1111/j.1530-0277.1996.tb05260.x


Author Worrall, S
deJersey, J
Wilce, PA
Seppa, K
Hurme, L
Sillanaukee, P
Title Relationship between alcohol intake and immunoglobulin A immunoreactivity with acetaldehyde-modified bovine serum albumin
Journal name Alcoholism-Clinical and Experimental Research   Check publisher's open access policy
ISSN 0145-6008
Publication date 1996-08-01
Year available 1996
Sub-type Article (original research)
DOI 10.1111/j.1530-0277.1996.tb05260.x
Open Access Status DOI
Volume 20
Issue 5
Start page 836
End page 840
Total pages 5
Place of publication BALTIMORE
Publisher WILLIAMS & WILKINS
Language eng
Abstract Acetaldehyde, the main metabolite of ethanol, is a highly reactive species that reacts with macromolecules to produce unstable and stable adducts. Acetaldehyde-modified proteins are immunogenic and have been detected in the liver and blood of alcoholics. Furthermore, antibodies reactive with acetaldehyde-modified proteins have been detected in the plasma of social drinkers and alcoholics. However, the class distribution of immunoglobulins reactive with modified proteins was different in the two groups, being predominantly immunoglobulin (Ig)M in social drinkers, but IgM and IgA in alcoholics. In this study, we demonstrate that heavy drinkers (alcohol intake >130 g/week for females and 150 g/week for males) also exhibit IgA reactivity with acetaldehyde-modified proteins. The IgA adduct-specific reactivity (IgA reactivity with acetaldehyde-modified bovine serum albumin-reactivity with native bovine serum albumin) showed a moderate correlation with self-reported alcohol intake, but did not correlate with markers such as plasma transaminase, gamma-glutamyltransferase activity, or mean corpuscular volume. IgA adduct-specific reactivity had similar specificity to the conventional tests of alcohol abuse, but had higher sensitivity than the other tests, especially with heavy drinkers. Data presented herein demonstrate that elevated IgA reactivity with acetaldehyde-modified epitopes is associated with heavy drinking and is a potential marker for high alcohol intake.
Keyword alcoholism
acetaldehyde
antibody formation
biological markers
enzyme-linked immunosorbent assay
Modified Epitopes
Liver-Disease
Antibodies
Consumption
Proteins
Adducts
Hemoglobin
Marker
Rats
Iga
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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