Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication

Mackenzie, JM, Jones, MK and Young, YR (1996) Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology, 220 1: 232-240. doi:10.1006/viro.1996.0307


Author Mackenzie, JM
Jones, MK
Young, YR
Title Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1096-0341
1089-862X
Publication date 1996-06-01
Year available 1996
Sub-type Article (original research)
DOI 10.1006/viro.1996.0307
Open Access Status DOI
Volume 220
Issue 1
Start page 232
End page 240
Total pages 9
Place of publication SAN DIEGO
Publisher ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Language eng
Subject 2406 Virology
Abstract Although many workers have investigated the maturation and processing of the flavivirus nonstructural glycoprotein NS1 in infected cells, these studies have provided little insight into a possible function for NS1. In this study we investigated the subcellular localization of NS1 both by immunofluorescence and Myo-immuno electron microscopy of infected Vero and C6/36 cells. NS1 was found to he tightly associated with intracellular membranes, in particular with Vesicle packets and targe cytoplasmic vacuoles. Surprisingly, NS1 did not associate with mature virus particles, a finding that is inconsistent with the postulated role for this protein in virion assembly and/or maturation. However, dual-labeling experiments did reveal in both the confocal immunofluorescence and cryo-immuno EM studies the colocalization of NS1 with the viral dsRNA replicative form. Furthermore, localization of the dsRNA to the vesicle packets and cytoplasmic vacuoles seen in infected Vero and C6/36 cells, respectively, suggests that these structures may comprise the flavivirus replication complex. These findings provide further insights into the maturation pathway of NS1 and suggest a possible role for this protein in viral RNA replication. (C) 1996 Academic Press, Inc.
Keyword Yellow-Fever Virus
Borne Encephalitis-Virus
Aedes-Albopictus Cells
Kunjin Virus
Monoclonal-Antibodies
Infected Vero
Protein Ns1
Form
Association
Microscopy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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