Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity

Meshulam, Tova, Breen, Michael R., Liu, Libin, Parton, Robert G. and Pilch, Paul F. (2011) Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity. Journal of Lipid Research, 52 8: 1526-1532. doi:10.1194/jlr.M015628

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Author Meshulam, Tova
Breen, Michael R.
Liu, Libin
Parton, Robert G.
Pilch, Paul F.
Title Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicity
Journal name Journal of Lipid Research   Check publisher's open access policy
ISSN 0022-2275
Publication date 2011-08-01
Year available 2011
Sub-type Article (original research)
DOI 10.1194/jlr.M015628
Open Access Status File (Publisher version)
Volume 52
Issue 8
Start page 1526
End page 1532
Total pages 7
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Abstract Mice and humans lacking functional caveolae are dyslipidemic and have reduced fat stores and smaller fat cells. To test the role of caveolins/caveolae in maintaining lipid stores and adipocyte integrity, we compared lipolysis in caveolin-1 (Cav1)-null fat cells to that in cells reconstituted for caveolae by caveolin-1 re-expression. We find that the Cav1-null cells have a modestly enhanced rate of lipolysis and reduced cellular integrity compared with reconstituted cells as determined by the release of lipid metabolites and lactic dehydrogenase, respectively, into the media. There are no apparent differences in the levels of lipolytic enzymes or hormonally stimulated phosphorylation events in the two cell lines. In addition, acute fasting, which dramatically raises circulating fatty acid levels in vivo, causes a significant upregulation of caveolar protein constituents. These results are consistent with the hypothesis that caveolae protect fat cells from the lipotoxic effects of elevated levels fatty acids, which are weak detergents at physiological pH, by virtue of the property of caveolae to form detergentresistant membrane domains.
Keyword Caveolin-1
Lipid rafts
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID R01 DK-56935
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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