The strong biocidal effect of free nitrous acid on anaerobic sewer biofilms

Jiang, Guangming, Gutierrez, Oriol and Yuan, Zhiguo (2011) The strong biocidal effect of free nitrous acid on anaerobic sewer biofilms. Water Research, 45 12: 3735-3743. doi:10.1016/j.watres.2011.04.026

Author Jiang, Guangming
Gutierrez, Oriol
Yuan, Zhiguo
Title The strong biocidal effect of free nitrous acid on anaerobic sewer biofilms
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
Publication date 2011-06-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.watres.2011.04.026
Open Access Status Not yet assessed
Volume 45
Issue 12
Start page 3735
End page 3743
Total pages 9
Place of publication Lisle, IL, United States
Publisher I W A Publishing
Language eng
Subject 2700 Medicine
2302 Ecological Modelling
2312 Water Science and Technology
2311 Waste Management and Disposal
2310 Pollution
Abstract Several recent studies showed that nitrite dosage to wastewater results in long-lasting reduction of the sulfate-reducing and methanogenic activities of anaerobic sewer biofilms. In this study, we revealed that the quick reduction in these activities is due to the biocidal effect of free nitrous acid (FNA), the protonated form of nitrite, on biofilm microorganisms. The microbial viability was assessed after sewer biofilms being exposed to wastewater containing nitrite at concentrations of 0–120 mg-N/L under pH levels of 5–7 for 6–24 h. The viable fraction of microorganisms was found to decrease substantially from approximately 80% prior to the treatment to 5–15% after 6–24 h treatment at FNA levels above 0.2 mg-N/L. The level of the biocidal effect has a much stronger correlation with the FNA concentration, which is well described by an exponential function, than with the nitrite concentration or with the pH level, suggesting that FNA is the actual biocidal agent. An increase of the treatment from 6 to 12 and 24 h resulted in only slight decreases in microbial viability. Physical disrupted biofilm was more susceptible to FNA in comparison with intact biofilms, indicating that the biocidal effect of FNA on biofilms was somewhat reduced by mass transfer limitations. The inability to achieve 2-log killing even in the case of disrupted biofilms suggests that some microorganisms may be more resistant to FNA than others. The recovery of biofilm activities in anaerobic reactors after being exposed to FNA at 0.18 and 0.36 mg-N/L, respectively, resembled the regrowth of residual sulfate-reducing bacteria and methanogens, further confirming the biocidal effects of FNA on microorganisms in biofilms.
Keyword Free nitrous acid
Sewer biofilm
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID LP0882016
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Advanced Water Management Centre Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 72 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 79 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 21 Aug 2011, 12:00:47 EST by System User on behalf of Advanced Water Management Centre