Expression and functional analysis of Dkk1 during early gonadal development

Combes, A. N., Bowles, J., Feng, C. -W., Chiu, H. S., Khoo, P. L., Jackson, A., Little, M. H., Tam, P. P. L. and Koopman, P. (2011) Expression and functional analysis of Dkk1 during early gonadal development. Sexual Development, 5 3: 124-130. doi:10.1159/000327709


Author Combes, A. N.
Bowles, J.
Feng, C. -W.
Chiu, H. S.
Khoo, P. L.
Jackson, A.
Little, M. H.
Tam, P. P. L.
Koopman, P.
Title Expression and functional analysis of Dkk1 during early gonadal development
Formatted title
Expression and functional analysis of Dkk1 during early gonadal development
Journal name Sexual Development   Check publisher's open access policy
ISSN 1661-5425
1661-5433
Publication date 2011-06-01
Year available 2011
Sub-type Article (original research)
DOI 10.1159/000327709
Open Access Status Not yet assessed
Volume 5
Issue 3
Start page 124
End page 130
Total pages 7
Place of publication Basel, Switzerland
Publisher S. Karger
Language eng
Abstract WNT signalling plays a central role in mammalian sex determination by promoting ovarian development and repressing aspects of testis development in the early gonad. Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT activity. Here, we examined the role of DKK1 in mouse sex determination and early gonadal development. Dkk1 mRNA was upregulated sex-specifically during testis differentiation, suggesting that DKK1 could repress WNT signalling in the developing testis. However, we observed overtly normal testis development in Dkk1-null XY gonads, and found no significant upregulation of Axin2 or Sp5 that would indicate increased canonical WNT signalling. Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors. Copyright (C) 2011 S. Karger AG, Basel
Formatted abstract
WNT signalling plays a central role in mammalian sex determination by promoting ovarian development and repressing aspects of testis development in the early gonad. Dickkopf homolog 1 (DKK1) is a WNT signalling antagonist that plays critical roles in multiple developmental systems by modulating WNT activity. Here, we examined the role of DKK1 in mouse sex determination and early gonadal development. Dkk1 mRNA was upregulated sex-specifically during testis differentiation, suggesting that DKK1 could repress WNT signalling in the developing testis. However, we observed overtly normal testis development in Dkk1-null XY gonads, and found no significant upregulation of Axin2 or Sp5 that would indicate increased canonical WNT signalling. Nor did we find significant differences in expression of key markers of testis and ovarian development. We propose that DKK1 may play a protective role that is not unmasked by loss-of-function in the absence of other stressors.
Keyword Dkk1
Rspo1
Testis development
Wnt4
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID DK070136
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
Institute for Molecular Bioscience - Publications
 
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