Menstrual effluent induces epithelial-mesenchymal transitions in mesothelial cells

Demir, A. Y., Groothuis, P. G., Nap, A. W., Punyadeera, C., de Goeij, A. F. P. M., Evers, J. L. H. and Dunselman, G. A. J. (2004) Menstrual effluent induces epithelial-mesenchymal transitions in mesothelial cells. Human Reproduction, 19 1: 21-29. doi:10.1093/humrep/deh042

Author Demir, A. Y.
Groothuis, P. G.
Nap, A. W.
Punyadeera, C.
de Goeij, A. F. P. M.
Evers, J. L. H.
Dunselman, G. A. J.
Title Menstrual effluent induces epithelial-mesenchymal transitions in mesothelial cells
Journal name Human Reproduction   Check publisher's open access policy
ISSN 0268-1161
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1093/humrep/deh042
Open Access Status
Volume 19
Issue 1
Start page 21
End page 29
Total pages 9
Place of publication Oxford United Kingdom
Publisher Oxford University Press
Language eng
Formatted abstract
Background: Menstrual effluent affects mesothelial cell (MC) morphology. We evaluated whether these changes were consistent with epithelial-mesenchymal transitions (EMT).
Methods: Monolayer cultures of MC were incubated overnight in conditioned media, prepared from cells isolated form menstrual effluent, with or without kinase and ATP inhibitors. Changes in cell morphology were monitored using time-lapse video microscopy and immunohistochemistry. Effects on the expression of EMT-associated molecules were evaluated using real-time RT-PCR and/or Western blot analysis. Results: Incubation in conditioned media disrupted cell-cell contacts, and increased MC motility. The changes were reversible. During the changes the distribution of cytokeratins, fibrillar actin and α-tubulin changed. Sodium azide, an inhibitor of ATP production, and Genistein, a general tyrosine kinase inhibitor, antagonized these effects. Wortmannin, a phosphatidylinositol 3-kinase inhibitor, and SU6656, an Src tyrosine kinase inhibitor, only partially antagonized the effect. The expression of Snail and vimentin was markedly up-regulated, whereas the expression of E-cadherin was decreased and cytokeratins were altered.
Conclusions: In MC, menstrual effluent initiates a reversible, energy-dependent transition process from an epithelial to a mesenchymal phenotype. Involvement of the (Src) tyrosine kinase signalling pathway and the changes in the expression of cytokeratins, Snail, vimentin and E-cadherin demonstrate that the morphological changes are EMT.
Keyword E-cadherin
Mesothelial cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 31 times in Thomson Reuters Web of Science Article | Citations
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