Three-dimensional flow-through protein platform

Van Lieshout, R. M. L., van Domburg, T., Saalmink, M., Verbeek, R., Wimberger-Friedl, R., van Dieijen-Visser, M. P. and Punyadeera, C. (2009) Three-dimensional flow-through protein platform. Analytical Chemistry, 81 13: 5165-5171. doi:10.1021/ac801244d

Author Van Lieshout, R. M. L.
van Domburg, T.
Saalmink, M.
Verbeek, R.
Wimberger-Friedl, R.
van Dieijen-Visser, M. P.
Punyadeera, C.
Title Three-dimensional flow-through protein platform
Journal name Analytical Chemistry   Check publisher's open access policy
ISSN 0003-2700
Publication date 2009-07-01
Sub-type Article (original research)
DOI 10.1021/ac801244d
Open Access Status
Volume 81
Issue 13
Start page 5165
End page 5171
Total pages 7
Place of publication Washington DC, United States
Publisher American Chemical Society
Language eng
Abstract We have developed a new protein microarray (Immuno-Flow Protein Platform, IFPP) that utilizes a porous nitrocellulose (NC) membrane with printed spots of capture probes. The sample is pumped actively through the NC membrane, to enhance binding efficiency and introduce stringency. Compared to protein microarrays assayed with the conventional incubation-shaking method the rate of binding is enhanced on the IFPP by at least a factor of 10, so that the total assay time can be reduced drastically without compromising sensitivity. Similarly, the sensitivity can be improved.We demonstrate the detection of 1 pM of C-reactive protein (CRP) in 70 μL of plasma within a total assay time of 7 min. The small sample and reagent volumes, combined with the speed of the assay, make our IFPP also well-suited for a point-of-care/near patient setting. The potential clinical application of the IFPP is demonstrated by validating CRP detection both in human plasma and serum samples against standard clinical laboratory methods.
Keyword C-Reactive Protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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