Tartrate resistant acid phosphatase activity in rat cultured osteoclasts is inhibited by a carboxyl terminal peptide (osteostatin) from parathyroid hormone-related protein

Zheng, M.H., McCaughan, H.B., Nicholson, G.C. and Wood, D.J. (1994) Tartrate resistant acid phosphatase activity in rat cultured osteoclasts is inhibited by a carboxyl terminal peptide (osteostatin) from parathyroid hormone-related protein. Journal of Cellular Biochemistry, 54 2: 145-153. doi:10.1002/jcb.240540203


Author Zheng, M.H.
McCaughan, H.B.
Nicholson, G.C.
Wood, D.J.
Title Tartrate resistant acid phosphatase activity in rat cultured osteoclasts is inhibited by a carboxyl terminal peptide (osteostatin) from parathyroid hormone-related protein
Journal name Journal of Cellular Biochemistry   Check publisher's open access policy
ISSN 0730-2312
1097-4644
Publication date 1994-02-01
Year available 1994
Sub-type Article (original research)
DOI 10.1002/jcb.240540203
Open Access Status Not yet assessed
Volume 54
Issue 2
Start page 145
End page 153
Total pages 9
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Formatted abstract
A carboxyl-terminal peptide sequence ('osteostatin') from parathyroid hormone related protein has been shown to have an inhibitory effect on osteoclastic bone resorption - an action opposite to its amino-terminal sequence. In this study, we proposed that inhibition of osteoclastic bone resorption by osteostatin was associated with reduction of tartrate resistant acid phosphatase (TRAcP) activity in osteoclasts. Our results have indicated that osteostatin reduced TRAcP activity in a dose dependent manner. This effect of osteostatin was both sensitive (half maximal effect approximately 5 x 10 -13 M) and potent (maximum inhibition approximately 50% of control). In the first 90 min of treatment, however, reduction of TRAcP activity was erratic but became persistent and progressive when the time course was extended. Moreover, throughout the experimental period the levels of TRAcP activity in the culture medium had fallen significantly. It appears that osteostatin has a biphasic effect on TRAcP activity, inhibiting its secretion and either suppressing its synthesis or increasing its degradation. In addition, osteostatin induced rapid cellular retraction of both human and rat cultured osteoclasts, which was morphologically distinct from that produced by calcitonin.
Keyword Osteoclast
Tartrate resistant acid phosphatase
Parathyroid hormone related protein
Osteocalcin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 19 February 2004.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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