Calcitonin receptors of human osteoclastoma

Nicholson, G.C., Sexton, P.M., Dsantos, C.S., Kemp, B.E. and Martin, T.J. (1987) Calcitonin receptors of human osteoclastoma. Hormone and Metabolic Research, 19 11: 585-589. doi:10.1055/s-2007-1011887

Author Nicholson, G.C.
Sexton, P.M.
Dsantos, C.S.
Kemp, B.E.
Martin, T.J.
Title Calcitonin receptors of human osteoclastoma
Journal name Hormone and Metabolic Research   Check publisher's open access policy
ISSN 0018-5043
Publication date 1987-11-01
Year available 1987
Sub-type Article (original research)
DOI 10.1055/s-2007-1011887
Open Access Status Not yet assessed
Volume 19
Issue 11
Start page 585
End page 589
Total pages 5
Place of publication Stuttgart, Germany
Publisher Georg Thieme
Language eng
Formatted abstract
Osteoclast-rich cultures were prepared by disaggregation of osteoclastomas (giant cell tumour of bone) and settlement onto glass or plastic surfaces. Autoradiography using [125I]-salmon calcitonin ([125I]-sCT) revealed specific binding only to multinucleate giant cells (osteoclasts) and a minor population of mononuclear cells. [125I]-sCT competitive binding studies indicated a K(d) of 5 x 10-10 M and receptor number of approximately 1 million sites/osteoclast. sCT treatment resulted in a dose-dependent rise in cAMP (EC50 10-10 M). Homogenates of an osteoclastoma also demonstrated specific binding of [125I]-sCT. Chemical cross-linking of a labelled synthetic sCT derivative, [125I]-[Arg11,18, Lys14]-sCT, using disuccinimidyl suberate, resulted in labelling of a receptor component of approximate M(r) 85-90,000. The multinucleate giant cells (osteoclasts) of human osteoclastomas possess large number of CT receptors which exhibit the same binding kinetics and apparent M(r) as those of other CT target cells.
Keyword Endocrinology & Metabolism
Endocrinology & Metabolism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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