Osteoblast-mediated effects of zinc on isolated rat osteoclasts: Inhibition of bone resorption and enhancement of osteoclast number

Holloway, W.R., Collier, .F.M., Herbst, R.E., Hodge, J.M. and Nicholson, G.C. (1996) Osteoblast-mediated effects of zinc on isolated rat osteoclasts: Inhibition of bone resorption and enhancement of osteoclast number. Bone, 19 2: 137-142. doi:10.1016/8756-3282(96)00141-X


Author Holloway, W.R.
Collier, .F.M.
Herbst, R.E.
Hodge, J.M.
Nicholson, G.C.
Title Osteoblast-mediated effects of zinc on isolated rat osteoclasts: Inhibition of bone resorption and enhancement of osteoclast number
Journal name Bone   Check publisher's open access policy
ISSN 8756-3282
Publication date 1996-08-01
Year available 1996
Sub-type Article (original research)
DOI 10.1016/8756-3282(96)00141-X
Open Access Status Not Open Access
Volume 19
Issue 2
Start page 137
End page 142
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Zinc is an important element in biology yet little is understood of its role in bone cell metabolism and function. This study examined the effects of zinc on osteoclast (OC) function in cultures derived from neonatal rats and in cocultures of OC and UMR 106-01 osteoblast-like cells (UMR/OC cocultures). Treatment with zinc (10 -12-10 -4 mol/L) had no effect on either bone resorption or the number of multinucleate cells positive for tartrate-resistant acid phosphatase (TRACP + ve MNC) in OC cultured for 24 h on bone slices. However, in UMR/OC cocultures, 10 -4 mol/L zinc (but not lower concentrations) decreased resorption pit formation by approximately 50% and increased TRACP + ve MNC number by approximately 40%. When osteoblast-like cells were pretreated with zinc prior to, but not during, coculture with OC, effects on TRACP + ve MNC and pit number persisted, although the effect was reduced. Zinc treatment also inhibited resorption and stimulated TRACP and calcitonin receptor (CTR) + ve MNC numbers in long-term (96-120 h) UMR/OC cocultures. Our results indicate that zinc increases TRACP + ve CTR + ve MNC numbers yet inhibits bone-resorbing activity, and that these effects are dependent on the presence of osteoblastic cells. Zinc is abundant in bone and may act as a local regulator of bone cells.
Keyword Zinc
Osteoclast
Osteoblast
Bone
Resorption
Coculture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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