Expression of functional RANK on mature rat and human osteoclasts

Myers, D.E., Collier, F.McL., Minkin, C., Wang, H., Holloway, W.R., Malakellis, M. and Nicholson, G.C. (1999) Expression of functional RANK on mature rat and human osteoclasts. FEBS Letters, 463 3: 295-300. doi:10.1016/S0014-5793(99)01650-6


Author Myers, D.E.
Collier, F.McL.
Minkin, C.
Wang, H.
Holloway, W.R.
Malakellis, M.
Nicholson, G.C.
Title Expression of functional RANK on mature rat and human osteoclasts
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
1873-3468
Publication date 1999-12-01
Sub-type Article (original research)
DOI 10.1016/S0014-5793(99)01650-6
Volume 463
Issue 3
Start page 295
End page 300
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Although the important roles of RANK/RANKL in osteoclastogenesis have been established, their roles in the regulation of mature osteoclasts remain uncertain. Microisolation has been used to obtain pure populations of rat and human osteoclasts for RT-PCR analysis. RANK and calcitonin receptor mRNA was detected in all the samples whereas OPG and ALP mRNA was not present in any. RANKL mRNA was detected in two of eight rat and one of four human samples. Treatment of osteoclasts with soluble RANKL resulted in translocation of NF- κB to the nucleus and elevation of cytosolic and nuclear calcium levels. We have shown that RANK is highly expressed in mature osteoclasts and that its stimulation by RANKL results in activation of NF-κB and calcium signalling.
Keyword Intracellular calcium
Microisolation
NF-kappaB
Osteoclast
RANK
RANKL
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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