C3d and the septal microvasculature as a predictor of chronic lung allograft dysfunction

Magro, Cynthia M., Abbas, Abbas E., Seilstad, Kay, Pope-Harman, Amy L., Nadasdy, Tibor and Ross, Jr., Patrick (2006) C3d and the septal microvasculature as a predictor of chronic lung allograft dysfunction. Human Immunology, 67 4-5: 274-283. doi:10.1016/j.humimm.2005.11.001

Author Magro, Cynthia M.
Abbas, Abbas E.
Seilstad, Kay
Pope-Harman, Amy L.
Nadasdy, Tibor
Ross, Jr., Patrick
Title C3d and the septal microvasculature as a predictor of chronic lung allograft dysfunction
Journal name Human Immunology   Check publisher's open access policy
ISSN 0198-8859
Publication date 2006-04-01
Sub-type Article (original research)
DOI 10.1016/j.humimm.2005.11.001
Open Access Status Not Open Access
Volume 67
Issue 4-5
Start page 274
End page 283
Total pages 10
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Studies have shown a potential role for humoral rejection in the evolution of lung graft dysfunction, apparently based on antibodies without human leukocyte antigen specificity. The correlation between extent of C4d deposition with clinical status further illustrates the importance of humoral immunity. Our study examines the potential value of C3d as a further diagnostic adjunct. C3d deposition was examined in lung allograft specimens using frozen tissue indirect direct immunofluorescence (IIF) and avidin biotin immunohistochemical applied to paraffin embedded tissue. Intermediate/extensive amounts of C3d using IIF and immunohistochemical (IH) methodologies correlated with chronic graft dysfunction; IIF C3d deposition was associated with septal and bronchial wall fibrosis (p < 0.0001). Weak/absent amounts of IIF and IH C3d correlated with clinical stability (p < 0.0001). Higher levels of C3d by IH were more sensitive than by IIF as a bronchiolitis obliterans syndrome determinant. C3d and C4d deposition using immunofluorescence and IH were correlated (p < 0.00001). C3d deposition appears prognostically significant. Higher tissue expression of C3d mark chronic graft dysfunction/persistent graft failure following transplantation. The close correlation between C3d and C4d lends credence to the role of humoral allograft rejection as a pulmonary graft dysfunction contributing factor. C3d by IH manifests higher sensitivity but similar specificity compared to C3d by IIF.
Keyword Lung transplantation
C3d deposition
Chronic graft dysfunction
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Issue: April-May 2006

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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