Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin

Mayavan, Sundar, Dutta, Naba K., Choudhury, Namita R., Kim, Misook, Elvin, Christopher M. and Hill, Anita J. (2011) Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin. Biomaterials, 32 11: 2786-2796. doi:10.1016/j.biomaterials.2010.12.030


Author Mayavan, Sundar
Dutta, Naba K.
Choudhury, Namita R.
Kim, Misook
Elvin, Christopher M.
Hill, Anita J.
Title Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin
Formatted title
Self-organization, interfacial interaction and photophysical properties of gold nanoparticle complexes derived from resilin-mimetic fluorescent protein rec1-resilin
Journal name Biomaterials   Check publisher's open access policy
ISSN 0142-9612
1878-5905
Publication date 2011-04-01
Sub-type Article (original research)
DOI 10.1016/j.biomaterials.2010.12.030
Open Access Status Not yet assessed
Volume 32
Issue 11
Start page 2786
End page 2796
Total pages 11
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
In this investigation we report the synthesis of optically coupled hybrid architectures based on a new biomimetic fluorescent protein rec1-resilin and nanometer-scale gold nanoparticles (AuNPs) in a one-step method using a non-covalent mode of binding protocol. The presence of uniformly distributed fluorophore sequences, eSer(Thr)-Tyr-Gly- along the molecular structure of rec1-resilin provides signnificant opportunity to synthesize fluorophore-modified AuNPs bioconjugates with unique photophysical properties. The detailed analyses of the AuNP-bioconjugates, synthesized under different experimental conditions using spectroscopic, microscopic and scattering techniques demonstrate the organizational pathways and the electronic and photophysical properties of the developed AuNP-rec1-resilin bioconjugates. The calculation of the bimolecular quenching constant using the SterneVolmer equation confirms that the dominant mechanism involved in quenching of fluorescence of rec1-resilin in the presence of AuNP is static. Photoacoustic infrared spectroscopy was employed to understand the nature of the interfacial interaction between the AuNP and rec1-resilin and its evolution with pH. In such bioconjugates the quenched emission of fluorescence by AuNP on the fluorophore moiety of rec1-resilin in the immediate vicinity of the AuNP has significant potential for fluorescence-based detection schemes, sensors and also can be incorporated into nanoparticle-based devices.
Keyword Biomimetic protein
Self-assembly
Gold-protein bioconjugates
Gold nanoparticles
Photophysical properties of bioconjugates
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 11 Apr 2011, 20:46:22 EST by Ms Misook Kim on behalf of School of Chemistry & Molecular Biosciences