Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle

De Jager, Nadia, Hudson, Nicolas J., Reverter, Antonio, Wang, Yong-Hong, Nagaraj, Shivashankar H., Cafe, Linda M., Greenwood, Paul L., Barnard, Ross T., Kongsuwan, Kritaya P. and Dalrymple, Brian P. (2011) Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle. Physiological Genomics, 43 9: 467-478. doi:10.1152/physiolgenomics.00226.2010


Author De Jager, Nadia
Hudson, Nicolas J.
Reverter, Antonio
Wang, Yong-Hong
Nagaraj, Shivashankar H.
Cafe, Linda M.
Greenwood, Paul L.
Barnard, Ross T.
Kongsuwan, Kritaya P.
Dalrymple, Brian P.
Title Chronic exposure to anabolic steroids induces the muscle expression of oxytocin and a more than fiftyfold increase in circulating oxytocin in cattle
Journal name Physiological Genomics   Check publisher's open access policy
ISSN 1094-8341
1531-2267
Publication date 2011-05-01
Sub-type Article (original research)
DOI 10.1152/physiolgenomics.00226.2010
Volume 43
Issue 9
Start page 467
End page 478
Total pages 12
Place of publication Bethesda, United States
Publisher American Physiological Society
Language eng
Abstract Molecular mechanisms in skeletal muscle associated with anabolic steroid treatment of cattle are unclear and we aimed to characterize transcriptional changes. Cattle were chronically exposed (68 ± 20 days) to a steroid hormone implant containing 200 mg trenbolone acetate and 20 mg estradiol (Revalor-H). Biopsy samples from 48 cattle (half treated) from longissimus dorsi (LD) muscle under local anesthesia were collected. Gene expression levels were profiled by microarray, covering 16,944 unique bovine genes: 121 genes were differentially expressed (DE) due to the implant (99.99% posterior probability of not being false positives). Among DE genes, a decrease in expression of a number of fat metabolism-associated genes, likely reflecting the lipid storage activity of intramuscular adipocytes, was observed. The expression of IGF1 and genes related to the extracellular matrix, slow twitch fibers, and cell cycle (including SOX8, a satellite cell marker) was increased in the treated muscle. Unexpectedly, a very large 21-(microarray) to 97 (real time quantitative PCR)-fold higher expression of the mRNA encoding the neuropeptide hormone oxytocin was observed in treated muscle. We also observed an ~50-fold higher level of circulating oxytocin in the plasma of treated animals at the time of biopsy. Using a coexpression network strategy OXTR was identified as more likely than IGF1R to be a major mediator of the muscle response to Revalor-H. A re-investigation of in vivo cattle LD muscle samples during early to mid-fetal development identified a > 128-fold increased expression of OXT, coincident with myofiber differentiation and fusion. We propose that oxytocin may be involved in mediating the anabolic effects of Revalor-H treatment.
Keyword Bovine
Gene expression
Hormone growth promotant
Longissimus dorsi
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published under "Call For Papers: Computational Modeling of Physiological Systems".

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2012 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 07 Apr 2011, 08:13:56 EST by Professor Ross Barnard on behalf of School of Chemistry & Molecular Biosciences