High pre-freezing dilution improves post-thaw function of ram spermatozoa

Leahy, T., Marti, J.I., Mendoza, N., Perez-Pe, R., Muino-Blanco, T., Cebrian-Perez, J.A., Evans, G. and Maxwell, W.M.C. (2010) High pre-freezing dilution improves post-thaw function of ram spermatozoa. Animal Reproduction Science, 19 1-2: 137-146. doi:10.1016/j.anireprosci.2009.12.003

Author Leahy, T.
Marti, J.I.
Mendoza, N.
Perez-Pe, R.
Muino-Blanco, T.
Cebrian-Perez, J.A.
Evans, G.
Maxwell, W.M.C.
Title High pre-freezing dilution improves post-thaw function of ram spermatozoa
Journal name Animal Reproduction Science   Check publisher's open access policy
ISSN 0378-4320
Publication date 2010-05-01
Sub-type Article (original research)
DOI 10.1016/j.anireprosci.2009.12.003
Volume 19
Issue 1-2
Start page 137
End page 146
Total pages 10
Place of publication The Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
Despite considerable cryobiology research there is no industry standard for the concentration to which ram spermatozoa should be diluted before freezing. Ram semen is highly concentrated and often frozen at a high sperm concentration, necessitating the use of small laparoscopic insemination doses. The aim of this paper was to ascertain the effect of dilution on the integrity of frozen-thawed ram spermatozoa. In the first experiment, spermatozoa were extended with a Tris-buffered diluent before freezing or after thawing to yield a final sperm concentration of 20 × 106/ml, or were not diluted. Motility characteristics, viability and acrosome integrity of spermatozoa were analysed over a 6 h incubation period at 37 °C. In the second experiment, spermatozoa were either diluted before freezing, subjected to sex-sorting or not diluted before freezing. Thawed spermatozoa were separated into sub-populations using centrifugal counter-current distribution (CCCD) and the profile of partition and functional integrity (viability, chlortetracycline status and Annexin-V binding) in the sub-populations assessed. Dilution before freezing significantly improved post-thaw viability, acrosome integrity and total motility whereas dilution post-thaw decreased viability and motility of spermatozoa. Sperm heterogeneity, as assessed by CCCD profile, was not different for control, diluted and sex-sorted spermatozoa. Analysis of CCCD sub-populations showed the proportion of functional cells (displaying the F-Pattern or no PS translocation) was similar for all sperm types. The results show that ram spermatozoa retain normal function at higher pre-freeze dilution rates than are commonly used in the sheep industry. The application of these findings would result in more practicable and functional artificial insemination doses. © 2009 Elsevier B.V. All rights reserved.
Keyword Cryopreservation
Dilution rate
Ram semen
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 05 Apr 2011, 00:06:45 EST by Dr Tamara Leahy on behalf of School of Veterinary Science