Chikungunya virus arthritis in adult wild-type mice

Gardner, Joy, Anraku, Itaru, Le, Thuy T., Larcher, Thibaut, Major, Lee, Roques, Pierre, Schroder, Wayne A., Higgs, Stephen and Suhrbier, Andreas (2010) Chikungunya virus arthritis in adult wild-type mice. Journal of Virology, 84 16: 8021-8032. doi:10.1128/JVI.02603-09

Author Gardner, Joy
Anraku, Itaru
Le, Thuy T.
Larcher, Thibaut
Major, Lee
Roques, Pierre
Schroder, Wayne A.
Higgs, Stephen
Suhrbier, Andreas
Title Chikungunya virus arthritis in adult wild-type mice
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2010-08-01
Year available 2010
Sub-type Article (original research)
DOI 10.1128/JVI.02603-09
Open Access Status DOI
Volume 84
Issue 16
Start page 8021
End page 8032
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Chikungunya virus is a mosquito-borne arthrogenic alphavirus that has recently reemerged to produce the largest epidemic ever documented for this virus. Here we describe a new adult wild-type mouse model of chikungunya virus arthritis, which recapitulates the self-limiting arthritis, tenosynovitis, and myositis seen in
humans. Rheumatic disease was associated with a prolific infiltrate of monocytes, macrophages, and NK cells and the production of monocyte chemoattractant protein 1 (MCP-1), tumor necrosis factor alpha (TNF-α), and gamma interferon (IFN-γ). Infection with a virus isolate from the recent Reunion Island epidemic induced significantly more mononuclear infiltrates, proinflammatory mediators, and foot swelling than did an Asian isolate from the 1960s. Primary mouse macrophages were shown to be productively infected with chikungunya virus; however, the depletion of macrophages ameliorated rheumatic disease and prolonged the viremia. Only 1 μg of an unadjuvanted, inactivated, whole-virus vaccine derived from the Asian isolate completely protected against viremia and arthritis induced by the Reunion Island isolate, illustrating that protection is not strain specific and that low levels of immunity are sufficient to mediate protection. IFN-α treatment was able to prevent arthritis only if given before infection, suggesting that IFN-α is not a viable therapy. Prior infection
with Ross River virus, a related arthrogenic alphavirus, and anti-Ross River virus antibodies protected mice against chikungunya virus disease, suggesting that individuals previously exposed to Ross River virus should be protected from chikungunya virus disease. This new mouse model of chikungunya virus disease thus provides insights into pathogenesis and a simple and convenient system to test potential new interventions.
Copyright © 2010, American Society for Microbiology. All Rights Reserved

Keyword Arthritis
Chikungunya virus
Ross River Virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 179 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 04 Apr 2011, 21:40:18 EST by Debbie Banks on behalf of School of Medicine