Novel single stranded DNA binding proteins crucial for the DNA damage response

Cubeddu, L., Richard, D. J., White, M. F. and Khanna, K. K. (2010) Novel single stranded DNA binding proteins crucial for the DNA damage response. The FEBS Journal, 277 Supp. 1: 193-193. doi:10.1111/j.1742-4658.2010.07680.x

Author Cubeddu, L.
Richard, D. J.
White, M. F.
Khanna, K. K.
Title Novel single stranded DNA binding proteins crucial for the DNA damage response
Journal name The FEBS Journal   Check publisher's open access policy
ISSN 1742-464X
Publication date 2010-06-01
Sub-type Other
DOI 10.1111/j.1742-4658.2010.07680.x
Open Access Status
Volume 277
Issue Supp. 1
Start page 193
End page 193
Total pages 1
Editor Richard Perham
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Single stranded DNA binding (SSB) proteins are ubiquitous and essential for a variety of DNA metabolic processes including DNA replication, recombination, transcription and repair as well as for the recruitment of the repair machinery to sites of DNA damage. SSB proteins from the three domains of life are evolutionarily conserved and utilise oligonucleotide-binding (OB) domains for DNA binding. We describe two new human SSBs (hSSB1 and 2), with a domain organisation closer to the archaeal SSB than to the eukaryotic Replication Protein A (RPA). Building on our recent data (Richard et al. (2008) Nature 453(7195): 677–681), we show that hSSB1 is critical for the cellular response to double-stranded DNA breaks (DSBs) and directly interacts with the MRN (Mre11/Rad50/NBS1) complex to facilitate the repair of lethal DSBs. Preliminary data indicate that the second SSB, hSSB2 is involved in the cellular response to ultra violet radiation and we have embarked on the first biochemical characterisation of this newly discovered protein. We propose that these novel hSSBs are functional homologues of the bacterial and archaeal SSB family of proteins, placing them centrally in the DNA repair pathway. Like many early participants in the damage response pathway, hSSBs may therefore be involved in tumorigenesis and may significantly affect the response of patients to cancer therapies.
© 2010 The Authors Journal compilation © 2010 Federation of European Biochemical Societies
Q-Index Code EX
Q-Index Status Confirmed Code
Institutional Status Non-UQ
Additional Notes Special Issue: Abstracts of the 35th FEBS (Federation of European Biochemical Societies) Congress, Gothenburg, Sweden, 26 June - 1 July 2010. Presented during and published under the Session "C1 – Molecular Machines" as Poster Abstract C1.16.

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