Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis

Nogueira, Lucas, Cardoso, Fernanda C., Mattos, Ana M., Bordignon, Juliano, Figueiredo, Cláudia P., Dahlstrom, Pedro, Frota, Cristiane C., Duarte dos Santos, Cláudia N., Chalhoub, Marcelo, Cavada, Benildo S., Teixeira, Henrique C., Oliveira, Sérgio C., Barral-Netto, Manoel and Báfica, André (2010) Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis. European Journal of Immunology, 40 3: 744-753. doi:10.1002/eji.200939747


Author Nogueira, Lucas
Cardoso, Fernanda C.
Mattos, Ana M.
Bordignon, Juliano
Figueiredo, Cláudia P.
Dahlstrom, Pedro
Frota, Cristiane C.
Duarte dos Santos, Cláudia N.
Chalhoub, Marcelo
Cavada, Benildo S.
Teixeira, Henrique C.
Oliveira, Sérgio C.
Barral-Netto, Manoel
Báfica, André
Title Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis
Formatted title
Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis
Journal name European Journal of Immunology   Check publisher's open access policy
ISSN 0014-2980
1521-4141
Publication date 2010-03-01
Year available 2010
Sub-type Article (original research)
DOI 10.1002/eji.200939747
Open Access Status DOI
Volume 40
Issue 3
Start page 744
End page 753
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley VCH Verlag
Language eng
Formatted abstract
In this study, we have identified a secreted 13 kDa lectin from Mtb (Mtb, Mycobacterium tuberculosis; sMTL-13) by homology search of a non-redundant lectin database. Bioinformatic analysis revealed that sMTL-13 belongs to the ricin-type β-trefoil family of proteins containing a Sec-type signal peptide present in Mtb complex species, but not in non-tuberculous mycobacteria. Following heterologous expression of sMTL-13 and generation of an mAb (clone 276.B7/IgG1κ), we confirmed that this lectin is present in culture filtrate proteins from Mtb H37Rv, but not in non-tuberculous mycobacteria-derived culture filtrate proteins. In addition, sMTL-13 leads to an increased IFN-γ production by PBMC from active tuberculosis (ATB) patients. Furthermore, sera from ATB patients displayed high titers of IgG Ab against sMTL-13, a response found to be decreased following successful anti-tuberculosis therapy. Together, our findings reveal a secreted 13 kDa ricin-like lectin from Mtb, which is immunologically recognized during ATB and could serve as a biomarker of disease treatment.
Keyword Biomarkers
Lectin
Mycobacterium tuberculosis
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 472477/2007-2
210/2007
04524/2008-1
2008-8734-0
Institutional Status Non-UQ
Additional Notes Article first published online: 16 DEC 2009

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 22 Mar 2011, 21:35:27 EST by Susan Allen on behalf of Institute for Molecular Bioscience