Bone marrow stromal cells are load responsive in vitro

Thomas, G.P. and ElHaj, A.J. (1996) Bone marrow stromal cells are load responsive in vitro. Calcified Tissue International, 58 2: 101-108. doi:10.1007/s002239900018


Author Thomas, G.P.
ElHaj, A.J.
Title Bone marrow stromal cells are load responsive in vitro
Journal name Calcified Tissue International   Check publisher's open access policy
ISSN 0171-967X
1432-0827
Publication date 1996-02-01
Year available 1996
Sub-type Article (original research)
DOI 10.1007/s002239900018
Open Access Status DOI
Volume 58
Issue 2
Start page 101
End page 108
Total pages 8
Place of publication New York, NY, United States
Publisher Springer New York
Language eng
Abstract Mechanical load-related effects on bone marrow stromal cellsin vitro have been investigated. A dose response of a cyclical load of 1 Hz between 350 ustrain and 2500 ustrain applied to 10-day-old cultures resulted in elevated alkaline phosphatase levels and the number of cells expressing this protein after 2 days. No significant changes in the number of cells expressing or the production of collagen type I was observed. A critical stage of development of the cultures must be reached before load-related elevation in alkaline phosphatase expression could be measured independent of the stage at which loading was applied. Using a prostaglandin inhibitor at concentrations previously usedin vivo, the load response was abolished. We have demonstrated that bone marrow stromal cells are load responsive in culture and have made preliminary studies into determining the involvement of prostaglandins in this process.
Keyword Bone marrow
Mechanical load
Differentiation
Prostaglandins
In vitro
Bone cells
Alkaline phosphatase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Mon, 21 Mar 2011, 22:33:39 EST