Species-divergent regulation of human and mouse osteocalcin genes by calciotropic hormones

Thomas, G.P., Bourne, A., Eisman, J.A. and Gardiner, E.M. (2000) Species-divergent regulation of human and mouse osteocalcin genes by calciotropic hormones. Experimental Cell Research, 258 2: 395-402. doi:10.1006/excr.2000.4912


Author Thomas, G.P.
Bourne, A.
Eisman, J.A.
Gardiner, E.M.
Title Species-divergent regulation of human and mouse osteocalcin genes by calciotropic hormones
Journal name Experimental Cell Research   Check publisher's open access policy
ISSN 0014-4827
1090-2422
Publication date 2000-08-01
Sub-type Article (original research)
DOI 10.1006/excr.2000.4912
Volume 258
Issue 2
Start page 395
End page 402
Total pages 8
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Formatted abstract
Although osteocalcin is the most abundant noncollagenous protein in bone, its role remains undefined. Recent studies have reported diametrically opposing responses in the vitamin D regulation of the mouse vs the human and rat osteocalcin genes. The aim of this study was to increase the understanding of these differences and further elucidate the physiological function and regulation of osteocalcin. Direct comparison of the regulation of both the endogenous mouse osteocalcin gene (mOC) and a human osteocalcin promoter-chloramphenicol acetyl transferase (hOC-CAT) reporter as integrated templates was undertaken in primary osteoblastic cultures from OSCAT transgenic mice. Expression of both genes was up-regulated with the onset of mineralization. Long-term chronic 1,25- dihydroxyvitamin D 3 (1,25-(OH) 2D 3) treatment and acute (2 day) PTH treatment inhibited both mOC and hOC-CAT expression. At all stages of osteoblastic development studied, hOC-CAT was up-regulated by acute 1,25-(OH) 2D 3, whereas mOC was unaffected or inhibited. Mouse osteopontin was strongly up-regulated by acute 1,25- (OH) 2D 3 treatment. Thus, the divergence of the osteocalcin responses to 1,25-(OH) 2D 3 is specific for the osteocalcin gene and for an acute 1,25- (OH) 2D 3 treatment regime. Elucidation of this unique aspect of bone physiology will provide valuable insights into the still incompletely understood roles of osteocalcin and 1,25-(OH) 2D 3 in bone.
Keyword Primary osteoblast
Osteocalcin
Mouse
Human
Calcitriol
OSCAT
Osteopontin
PTH
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Mon, 21 Mar 2011, 22:33:31 EST