Altered fatty acid metabolism in long duration road transport: An NMR-based metabonomics study in sheep

Li, Juan, Wijffels, Gene, Yu, Yihua, Nielsen, Lars K., Niemeyer, Dominic O., Fisher, Andrew D., Ferguson, Drewe M. and Schirra, Horst Joachim (2011) Altered fatty acid metabolism in long duration road transport: An NMR-based metabonomics study in sheep. Journal of Proteome Research, 10 3: 1073-1087. doi:10.1021/pr100862t


Author Li, Juan
Wijffels, Gene
Yu, Yihua
Nielsen, Lars K.
Niemeyer, Dominic O.
Fisher, Andrew D.
Ferguson, Drewe M.
Schirra, Horst Joachim
Title Altered fatty acid metabolism in long duration road transport: An NMR-based metabonomics study in sheep
Journal name Journal of Proteome Research   Check publisher's open access policy
ISSN 1535-3893
1535-3907
Publication date 2011-03-04
Year available 2010
Sub-type Article (original research)
DOI 10.1021/pr100862t
Open Access Status Not yet assessed
Volume 10
Issue 3
Start page 1073
End page 1087
Total pages 15
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Subject 1303 Biochemistry
1600 Chemistry
Abstract The physical, endocrine, and metabolic responses of livestock to road transport have been evaluated by conventional hematological and biochemistry parameters for more than 20 years. However, these measures are relatively insensitive to subtle metabolic adaptations. We applied NMR-based metabonomics to assess system-wide metabolic responses as expressed in urine and serum of a large cohort of animals (n = 80) subjected to 12 and 48 h road transport. The profiling of H NMR spectra revealed that the transported animals experienced altered gut and energy metabolism, muscle catabolism, and possibly a renal response. The animals transported for 48 h exhibited a deeper metabolic response to the transport event and a complex and expanded metabolic trajectory over the 72 h recovery period. Intriguingly, excretion of acyl glycines and a dicarboxylic acid was observed after transport and during recovery, implicating peroxisomal fatty acid oxidation as a metabolic response to transport-induced stress.
Formatted abstract
The physical, endocrine, and metabolic responses of livestock to road transport have been evaluated by conventional hematological and biochemistry parameters for more than 20 years. However, these measures are relatively insensitive to subtle metabolic adaptations. We applied NMR-based metabonomics to assess system-wide metabolic responses as expressed in urine and serum of a large cohort of animals (n = 80) subjected to 12 and 48 h road transport. The profiling of 1H NMR spectra revealed that the transported animals experienced altered gut and energy metabolism, muscle catabolism, and possibly a renal response. The animals transported for 48 h exhibited a deeper metabolic response to the transport event and a complex and expanded metabolic trajectory over the 72 h recovery period. Intriguingly, excretion of acyl glycines and a dicarboxylic acid was observed after transport and during recovery, implicating peroxisomal fatty acid oxidation as a metabolic response to transport-induced stress.
© 2010 American Chemical Society.
Keyword Metabonomics
Transport
Stress
Fasting
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Publication Date (Web): December 13, 2010.

 
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