A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation

Schwarz, Flavio, Huang, Wei, Li, Cishan, Schulz, Benjamin L., Lizak, Christian, Palumbo, Alessandro, Numao, Shin, Neri, Dario, Aebi, Markus and Wang, Lai-Xi (2010) A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation. Nature Chemical Biology, 6 4: 264-266. doi:10.1038/NCHEMBIO.314


Author Schwarz, Flavio
Huang, Wei
Li, Cishan
Schulz, Benjamin L.
Lizak, Christian
Palumbo, Alessandro
Numao, Shin
Neri, Dario
Aebi, Markus
Wang, Lai-Xi
Title A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation
Journal name Nature Chemical Biology   Check publisher's open access policy
ISSN 1552-4450
Publication date 2010-04-01
Year available 2010
Sub-type Article (original research)
DOI 10.1038/NCHEMBIO.314
Open Access Status Not yet assessed
Volume 6
Issue 4
Start page 264
End page 266
Total pages 3
Place of publication United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins. © 2010 Nature America, Inc. All rights reserved.
Keyword Biochemistry & Molecular Biology
Biochemistry & Molecular Biology
BIOCHEMISTRY & MOLECULAR BIOLOGY
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 3100AQ-105541
GM080374
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 18 Mar 2011, 22:08:48 EST by Mr Benjamin Schulz on behalf of School of Chemistry & Molecular Biosciences