A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers

Edwards, Anne, Fulton, Daniel C., Hylton, Christopher M., Jobling, Stephen A., Gidley, Michael, Rossner, Ute, Martin, Cathie and Smith, Alison M. (1999) A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers. Plant Journal, 17 3: 251-261. doi:10.1046/j.1365-313X.1999.00371.x


Author Edwards, Anne
Fulton, Daniel C.
Hylton, Christopher M.
Jobling, Stephen A.
Gidley, Michael
Rossner, Ute
Martin, Cathie
Smith, Alison M.
Title A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers
Journal name Plant Journal   Check publisher's open access policy
ISSN 0960-7412
1365-313X
Publication date 1999-01-01
Sub-type Article (original research)
DOI 10.1046/j.1365-313X.1999.00371.x
Volume 17
Issue 3
Start page 251
End page 261
Total pages 11
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract A chimeric antisense construct has been used to generate transgenic potatoes (Solanum tuberosumL.) in which activities of both of the main starch synthases responsible for amylopectin synthesis in the tuber (SSII and SSIII) are reduced. The properties of starch from tubers of these plants have been compared with those of starches from transgenic plants in which activity of either SSII or SSIII has been reduced. Starches from the three types of transgenic plant are qualitatively different from each other and from the starch of control plants with unaltered starch synthase activities, with respect to granule morphology, the branch lengths of amylopectin, and the gelatinisation behaviour analysed by viscometry. The effects of reducing SSII and SSIII together cannot be predicted from consideration of the effects of reducing these two isoforms individually. These results indicate that different isoforms of starch synthase make distinct contributions to the synthesis of amylopectin, and that they act in a synergistic manner, rather than independently, during amylopectin synthesis.
Keyword Pisum sativum-L
Granule
Pea
Crystalline
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Article first published online: 5 January 2002.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 17 Mar 2011, 06:31:14 EST