Cryopreserved human bone marrow stroma is fully functional in vitro

Nicol, Andrew, Nieda, Mie, Donaldson, Craig, Denning-Kendall, Patricia, Truman, Carol, Bradley, Ben and Hows, Jill (1996) Cryopreserved human bone marrow stroma is fully functional in vitro. British Journal of Haematology, 94 2: 258-265. doi:10.1046/j.1365-2141.1996.d01-1812.x

Author Nicol, Andrew
Nieda, Mie
Donaldson, Craig
Denning-Kendall, Patricia
Truman, Carol
Bradley, Ben
Hows, Jill
Title Cryopreserved human bone marrow stroma is fully functional in vitro
Journal name British Journal of Haematology   Check publisher's open access policy
ISSN 0007-1048
Publication date 1996-08-01
Sub-type Article (original research)
DOI 10.1046/j.1365-2141.1996.d01-1812.x
Volume 94
Issue 2
Start page 258
End page 265
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Abstract Human marrow long-term culture (LTC) enables maintenance of both stromal and haemopoietic elements of normal bone marrow (NBM) in vitro for 4-6 months. Stroma-based cultures are critical for quantitation of long-term culture initiating cells (LTC-IC), the most primitive human haemopoietic cells measurable in vitro. Supply of NBM can be sporadic, and up to 3-4 weeks in culture is required for stromal maturity. Stroma availability for experimental purposes can therefore be limited. Efforts to produce transformed human and transfected murine stromal cell lines comparable to NBM stroma have had some success. As an alternative, we investigated cryopreserved NBM and cryopreserved preformed stroma. Function of cryopreserved and control fresh NBM stroma was similar when evaluated for up to 12 weeks in LTC. We have also demonstrated that stroma derived from cryopreserved NBM or preformed cryopreserved NBM stroma can sustain third-party haemopoiesis as efficiently as fresh NBM stroma in LTC. Batched cryopreserved stroma is a convenient, rapidly available, source of functional stroma which avoids the logistic difficulties and lack of standardization associated with stroma from fresh NBM. This important advance will enhance the use of stroma-based LTC in studies of human haemopoiesis.
Keyword Long term culture initiating cells
Bone marrow stroma
Cord blood
Limiting dilution assay
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article first published online: 30 September 2008.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Created: Tue, 15 Mar 2011, 21:30:54 EST