Distribution of apoA-I-containing HDL subpopulations in patients with coronary heart disease

Asztalos, B. F., Roheim, P. S., Milani, R. L., Lefevre, M., McNamara, J. R., Horvath, K. V. and Schaefer, E. J. (2000) Distribution of apoA-I-containing HDL subpopulations in patients with coronary heart disease. Arteriosclerosis, Thrombosis, and Vascular Biology, 20 12: 2670-2676.

Author Asztalos, B. F.
Roheim, P. S.
Milani, R. L.
Lefevre, M.
McNamara, J. R.
Horvath, K. V.
Schaefer, E. J.
Title Distribution of apoA-I-containing HDL subpopulations in patients with coronary heart disease
Journal name Arteriosclerosis, Thrombosis, and Vascular Biology   Check publisher's open access policy
ISSN 1079-5642
Publication date 2000-12-01
Year available 2000
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 20
Issue 12
Start page 2670
End page 2676
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Lippincott Williams & Wilkins
Language eng
Formatted abstract
High density lipoproteins (HDLs) and their subspecies play a role in the development of coronary heart disease (CHD). HDL subpopulations were measured by 2-dimensional nondenaturing gel electrophoresis in 79 male control subjects and 76 male CHD patients to test the hypothesis that greater differences in apolipoprotein (apo)A-I-containing HDL subpopulations would exist between these 2 groups than for traditional lipid levels. In CHD subjects, HDL cholesterol (HDL-C) was lower (-14%, P<0.001), whereas total cholesterol and the low density lipoprotein cholesterol/HDL-C ratio were higher (9% [P<0.05] and 21% [P<0.01], respectively) compared with control levels. No significant differences were found for low density lipoprotein cholesterol, triglyceride, and apoA-I levels. In CHD subjects, there were significantly (P<0.001) lower concentrations of the large lipoprotein (Lp)A-I α1 (-35%), pre-α, (-50%), pre-α2 (-33%), and pre-α3 (-31%) subpopulations, whereas the concentrations of the small LpA-I/A-II α3 particles were significantly (P<0.001) higher (20%). Because α1 was decreased more than HDL-C and plasma apoA-1 concentrations in CHD subjects, the ratios of HDL-C to α1 and of apoA-I to α1 were significantly (P<0.001) higher by 36% and 57%, respectively, compared with control values. Subjects with low HDL-C levels (≤35 mg/dL) have different distributions of apoA-I-containing HDL subpopulations than do subjects with normal HDL-C levels (>35 mg/dL). Therefore, we stratified participants according to HDL-C concentrations into low and normal groups. The differences in lipid levels between controls and HDL-C-matched cases substantially decreased; however, the significant differences in HDL subspecies remained. Our research findings support the concept that compared with control subjects, CHD patients not only have HDL deficiency but also have a major rearrangement in the HDL subpopulations with significantly lower α1 and pre-α1-3 (LpA-I) and significantly higher α3 (LpA-I/A-II) particles.
Keyword HDL subpopulations
Coronary heart disease
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID HL-56160
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 151 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 14 Mar 2011, 19:45:24 EST