Distribution and characterisation of rat choroidal mast cells

Steptoe, R. J., McMenamin, P. G. and McMenamin, C. (1994) Distribution and characterisation of rat choroidal mast cells. British Journal of Ophthalmology, 78 3: 211-218. doi:10.1136/bjo.78.3.211


Author Steptoe, R. J.
McMenamin, P. G.
McMenamin, C.
Title Distribution and characterisation of rat choroidal mast cells
Journal name British Journal of Ophthalmology   Check publisher's open access policy
ISSN 0007-1161
Publication date 1994-03-01
Year available 1994
Sub-type Article (original research)
DOI 10.1136/bjo.78.3.211
Open Access Status DOI
Volume 78
Issue 3
Start page 211
End page 218
Total pages 8
Place of publication LONDON
Publisher BRITISH MED JOURNAL PUBL GROUP
Language eng
Abstract Despite the implication that choroidal mast cells are involved in the onset of experimental autoimmune uveoretinitis (EAU), a widely used animal model of uveoretinitis, little is known of these cells. In the present study the distribution, total number, regional density, and phenotype of choroidal mast cells were examined in Lewis, Wistar Furth, PVG/c, and brown Norway rats. Choroidal mast cells were predominantly associated with arteries and arterioles of more than 30 mu m diameter which lie in the outer (sclerad) choroid. The density of mast cells was greatest in the posterior choroid with density diminishing anteriorly. The choroid of male Lewis rats contained significantly greater number of mast cells than that of females (p<0.01). Histochemical (Alcian blue/safranin) and immunohistochemical (anti-rat mast cell protease I and II monoclonal antibodies) studies revealed choroidal mast cells were of the connective tissue type. However, granule proteinase content appeared less than that of well characterised connective tissue mast cell populations such as those in mesentery and skin. Lewis rats exhibited the highest density of choroidal mast cells (23.6 (SD 1.2)/mm(2)), Wistar Furth approximately half that of Lewis (13.5 (0.7)/mm(2)) while PVG/c and brown Norway rats had very low densities (3.06 (0.3); 1.95 (0.2)/mm(2) respectively). These studies provide valuable choroidal mast cell data for rats which may have implications for our understanding of experimental models of intraocular inflammation and clinical uveitis.
Keyword RESPIRATORY-TRACT
DENDRITIC CELLS
HARDERIAN-GLAND
SERUM IGE
HETEROGENEITY
RESPONSES
ANTIGEN
HAMSTER
RADIOIMMUNOASSAY
LOCALIZATION
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 16 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 11 Mar 2011, 23:31:43 EST