Mobilization of hematopoietic stem cells by depleting bone marrow macrophages

Barbier, Valerie, Winkler, Ingrid G. and Levesque, Jean-Paul (2012). Mobilization of hematopoietic stem cells by depleting bone marrow macrophages. In Stem cell mobilization: methods and protocols (pp. 117-137) New York, United States: Humana Press. doi:10.1007/978-1-61779-943-3_11

Author Barbier, Valerie
Winkler, Ingrid G.
Levesque, Jean-Paul
Title of chapter Mobilization of hematopoietic stem cells by depleting bone marrow macrophages
Title of book Stem cell mobilization: methods and protocols
Place of Publication New York, United States
Publisher Humana Press
Publication Year 2012
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-61779-943-3_11
Series Methods in Molecular Biology
ISBN 9781617799426
ISSN 1064-3745
Volume number 904
Chapter number 11
Start page 117
End page 137
Total pages 21
Total chapters 20
Language eng
Abstract/Summary An important factor contributing to hematopoietic stem cell (HSC) mobilization is the ability of mobilizing cytokines and chemotherapy to disturb the cellular components of HSC niches, particularly osteoblasts and their progenitors, and to inhibit the production of HSC supportive cytokines and chemokines. Although the mechanisms by which niche cells are inhibited by mobilizing treatments is still incompletely understood, it has recently emerged that bone marrow macrophages play a critical role in maintaining osteoblasts, bone formation, and the expression of CXCL12, KIT ligand, and angiopoietin-1 necessary to HSC maintenance. In this chapter, we describe how to mobilize HSC into the blood in mice by depleting macrophages with clodronate-loaded liposomes and compare this mode of mobilization to mobilization induced by granulocyte colony-stimulating factor and cyclophosphamide. Detailed methods to analyze mobilization of phenotypic and functional reconstituting HSC are described with examples.
Keyword Hematopoietic stem cells
Bone marrow
Stem cell niche
Depletion of macrophages
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status Non-UQ

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Created: Thu, 10 Mar 2011, 19:37:44 EST by Debbie Banks on behalf of School of Medicine