Multiple, coordinated Ca2+-release events underlie the inositol trisphosphate-induced local Ca2+ spikes in mouse pancreatic acinar cells

Thorn, P., Moreton, R. and Berridge, M. (1996) Multiple, coordinated Ca2+-release events underlie the inositol trisphosphate-induced local Ca2+ spikes in mouse pancreatic acinar cells. EMBO Journal, 15 5: 999-1003.

Author Thorn, P.
Moreton, R.
Berridge, M.
Title Multiple, coordinated Ca2+-release events underlie the inositol trisphosphate-induced local Ca2+ spikes in mouse pancreatic acinar cells
Journal name EMBO Journal   Check publisher's open access policy
ISSN 0261-4189
Publication date 1996-03-01
Year available 1996
Sub-type Article (original research)
Volume 15
Issue 5
Start page 999
End page 1003
Total pages 5
Place of publication London, United Kingdom
Publisher Nature
Language eng
Formatted abstract
Ca2+ wave initiation and non-propagating Ca2+ spikes occur as a result of localized Ca2+ release from the more sensitive intracellular Ca2+ stores. Using high spatial and temporal Ca2+ -imaging techniques we have investigated inositol 1,4,5 triphosphate (InsP3)-induced local Ca2+ spiking, which occurs at the site of Ca2+ wave initiation in pancreatic acinar cells. The spatial and temporal organization of a single spike suggested discrete hot spots of Ca2+ release. Further analysis of long trains of Ca2+ spikes demonstrated that these hot spots showed regenerative Ca2+ -release events which were consistently active from spike to spike. Regions adjacent to these hot spots also showed regenerative Ca2+ -release events of similar amplitude but with a much lower frequency of occurrence. We conclude that the InsP3-induced non-propagating Ca2+ spikes can be devolved into smaller components of release. Our results are consistent with a model of coordinated activity of pacemaker hot spots of Ca2+ release that recruit and entrain active Ca2+ -release events from surrounding regions.
Keyword Calcium signalling
InsP(3)
Pancreatic acinar cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Thu, 10 Mar 2011, 18:47:58 EST